A loop-mediated isothermal amplification (LAMP) assay was developed to detect the sexually-transmitted parasite, Trichomonas vaginalis in vaginal swabs. The presence of T. vaginalis was detected from 121 female sex workers attending a social hygiene clinic in Balibago, Angeles City, Pampanga, Philippines using culture, polymerase chain reaction (PCR), and the developed LAMP assay. The high anal...

Ro 7-0207 is a light yellow, crystalline substance with a melting range of 74-79oC. The pH of a 1 per cent. aqueous solution is about 6-6. In water its solubility is 2-4 per cent. at 25°C. In 95 per cent. ethanol its solubility is > 50 per cent. at 25GC. Aqueous solutions (0-5 per cent.) are relatively stable upon heating at 100GC. for 1 hr. except at pH values above 7. Ro 7-0207 is an effectiv...

A series of mono- and bis-salicylaldimine ligands and their corresponding Rh(i) complexes were prepared. The compounds were characterised using standard spectroscopic techniques including NMR, IR spectroscopy and mass spectrometry. The salicylaldimine ligands and complexes were screened for antiparasitic activity against two strains of Plasmodium falciparum i.e. the NF54 CQ-sensitive and K1 CQ-...

Clinical isolates of Trichomonas vaginalis were obtained from women consecutively enrolled in a study of partner notification. Testing of susceptibility to metronidazole and tinidazole was performed. Resistance to metronidazole and tinidazole was present in 17/178 (9.6%) and 1/178 (0.56%) strains, respectively. In vitro resistance was poorly correlated with clinical response to treatment.

Trichomoniasis may be diagnosed by a direct smear examination and by culture of the organism. Cultures are usually examined after 24 hrs' incubation and thereafter daily up to 7 days; this technique is known to detect some infections in which direct smears have been found to be negative. It was felt that some improvements could be made in the culture technique used for diagnosis and an investig...

Three hundred high vaginal swabs from pregnant women were cultured to investigate the vaginal flora during pregnancy. Two hundred swabs obtained from a private clinic were cultured aerobically for bacteria only, the remaining 100 from antenatal clinic of Khyber Hospital Peshawar were cultured both aerobically and anaerobically for bacteria and fungi (candida). Vaginal smears from patients in an...

Background

The diagnosis of Trichomonas vaginalis (Donne, 1837) infection by direct recognition of the parasite is comparatively easy in the acute phase in the female, more difficult in the male and in the female in the non-acute phase. Because of this difficulty conclusive epidemiological studies on T. vaginalis infection are few. Burch, Rees, and Reardon (1959) have contributed an informative study of t...

Trichomonas vaginalis is a globally common sexually transmitted human parasite. Many strains of T. vaginalis from around the world have been described to be resistant to the current drug of choice, metronidazole. However, only a few cases of metronidazole resistance have been reported from Europe. The resistant strains cause prolonged infections which are difficult to treat. T. vaginalis infect...