The cell wall fraction of the gram-positive, nontoxic Corynebacterium diphtheriae strain C8r(-) Tox- (=ATCC 11913) contained a channel-forming protein, as judged from reconstitution experiments with artificial lipid bilayer experiments. The channel-forming protein was present in detergent-treated cell walls and in extracts of whole cells obtained using organic solvents. The protein had an appar...

The effects of mineral deficiencies on plant growth have been studied for many years, but little use has been made of mineral deficiency as a tool for understanding the photosynthetic process. Hill reaction rates of chloroplasts from mineraldeficient plants were measured by Spencer and Possingham (1), who found lower rates per unit of chlorophyll in all deficiencies except iron. Kessler's (9) s...

BACKGROUND Human T lymphocytes infiltrating tissues in autoimmune diseases are known to express somatostatin receptors amongst other activation markers. In this study, we evaluated whether somatostatin receptor scintigraphy (SRS) using a radiolabelled somatostatin analogue ((99m)Tc-EDDA/tricine-HYNIC-tyr(3)-octreotide ((99m)Tc-EDDA/HYNIC-TOC)) is able to detect the presence of immune-mediated p...

By polyacrylamide gel electrophoresis, DEAE Sephacel, and hydroxyapatite chromatography, one form of glutamine synthetase has been identified in spinach (Spinacia oleracea L. cv. Monstrueux de Viroflay) leaves. It is localized only inside the chloroplast. The enzyme was purified to homogeneity and specific antibodies against the protein were raised by immunization of rabbits. The intracellular ...

The quinic acid ulitization (qut) pathway in Aspergillus nidulans is a dispensable carbon utilization pathway that catabolizes quinate to protocatechuate via dehydroquinate and dehydroshikimate(DHS). At the usual in vitro growth pH of 6.5, quinate enters the mycelium by means of a specific permease and is converted into PCA by the sequential action of the enzymes quinate dehydrogenase, 3-dehydr...

Antimicrobial peptides are an abundant group of proteinaceous compounds widely produced in the plant kingdom. Here, we report the purification and partial characterization of a novel antimicrobial peptide compound from horseradish tissue culture (Armoracia rusticana), named Ar-AMP, with bactericidal activity against both Gram-positive and Gram-negative bacteria. The production of antimicrobial ...

Many methods using polymerase chain reaction (PCR) have been developed and modified to isolate an unknown DNA sequence adjacent to a known sequence; these are collectively called gene-walking methods (1,4–9). However, their usage has been restricted because most previous methods had practical problems, including low sensitivity, low efficiency (small probability of success) and/or are laborious...

Previous experiments in this laboratory have revealed that the action spectrum of light-induced proton uptake of spinach chloroplasts stimulated by reduced trimethylbenzoquinone (TMQH2) has a pronounced shoulder in the 710-mj region.' Preliminary evidence indicated that the quantum efficiency of H+ uptake at wavelengths beyond 700 m1A might be greater than that for cytochrome c photoreduction u...