The activation of glycogen phosphorylase by epinephrine was examined in hepatocytes from fed female rats administered 0.25 mg of triiodothyronine per kg of body weight daily for 2 days. Basal cyclic AMP accumulation by the hepatocytes was not altered by the thyroid hormone administration. Basal glycogen phosphorylase a activities were 1.18 f 0.07 units/mg of protein in control and 0.37 -+ 0.04 ...

Thyroxine (T4), triiodothyronine (T3) and reverse triodothyronine (rT3) concentrations in human milk were measured by radioimmunoassay in 114 samples obtained from 1 week to 8 months postpartum. Several assay systems applied for the determination of serum thyroid hormone concentration were proved to be unsuitable for human milk, and the method of separating free and antibody-bound hormone by po...

We developed a thyroid hormone (TH) inducible primary screening assay for the identification and assessment of man-made chemicals that interfere with the TH-signalling pathway within target cells. The assay was developed in a Xenopus laevis cell line that was transduced with a self-inactivating (SIN) lentivirus vector (LV) containing a luciferase gene. The luciferase activation in this cell lin...

Hypothyroidism There is general agreement that all patients with symptomatic hypothyroidism should receive replacement treatment. MANAGEMENT Thyroxine should be used in the routine management of hypothyroidism. The initial dose should not exceed 0-05 mg daily in patients over 40; an initial dose of 0-1 mg daily may be used in younger patients. The dose should be increased by increments until th...

The effect of temperature on the binding of thyroxine and triiodothyronine to thyroxine-binding globulin has been studied by equilibrium dialysis. Inclusion of ovalbumin in the dialysis mixture stabilized thyroxine-binding globulin against losses in binding activity which had been found to occur during equilibrium dialysis. Ovalbumin by itself bound the thyroid hormones very weakly and its bind...