N(2-Thiophenyl)-1-(2-hydoxyphenyl)Imine (NTPHPI) was used as a proper complexing agent for the preconcentration by cloud point extraction followed by atomic absorption spectrometric to determine of Ni, Cu and Co ions in real samples. NTPHPI and Triton X-114 were used as hydrophobic ligand and non-ionic surfactant respectively and the pH of 8 was chosen as optimum. Under the optimized experiment...

Spontaneous enzymic release of renal dipeptidase (RDPase; EC 3.4.13.19), a glycosylphosphatidylinositol (GPI)-linked ectoenzyme, was observed in vitro during incubation of porcine proximal tubules at 37 degrees C. Triton X-114 phase separation of the released RDPase showed that the majority of the enzyme activity partitioned into the aqueous phase, indicating its hydrophilic nature. Immunoblot ...

Reference 1. Theilacker C, Diederich A-K, Otto A, Sava IG, Wobser D, Bao Y, et al. (2015) Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response. PLoS ONE 10(7): e0132949. doi: 10.1371/journal.pone.0132949 PMID: 26172831 Fig 3. Stimulation of TNF-α production in RAW 264.7 mousemacrophages by lipoprotein-enriched cell membrane fract...

The ability of Trypanosoma cruzi to interact with plasminogen, the zimogenic form of the blood serin protease plasmin, was examined. Immunohistochemistry studies revealed that both forms, epimastigotes and metacyclic trypomastigotes, were able to fix plasminogen in a lysine dependant manner. This interaction was corroborated by plasminogen activation studies. Both forms of the parasite enhanced...

Mammalian hck, a member of the src family of tyrosine kinases, is expressed predominantly in cells of the myeloid and B-lymphoid lineages. Using mutational analysis, we have investigated the molecular basis of two immunoreactive forms of murine hck of 56 and 59 kDa found in numerous hemopoietic cell types. Our results indicate that translation of murine p59hck initiates from a CTG codon located...

The objective of this study was to partially purify ß-glucanase from crude enzyme extract of straw mushroom (Volvariella volvacea). The crude enzyme extract was prepared from the fruiting bodies of straw mushroom. The ßglucanase was partially purified using an aqueous two-phase extraction with Triton X-114 and followed by ammonium sulfate precipitation in the range of 50-80% saturation. The ß-g...

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. Herein, the characterization of VP24, a major structural protein of WSSV, is described. When purified virions were subjected to Nonidet P-40 treatment to separate the envelopes from the nucleocapsids, VP24 was found to be present exclusively in the envelope fraction. Triton X-114 extraction ...

The mitochondrial branched-chain alpha-keto acid transporter was solubilized from rat heart mitochondria and its function was reconstituted in phospholipid vesicles. The transporter was extracted from mitoplasts with Triton X-114 in the presence of exogenous cardiolipin and alpha-ketoisocaproate. Upon incorporation of this extract into asolectin vesicles by the freeze-thaw-sonication technique,...

Triton X-100, as one of the most popular surfactants used in bioremediation techniques, has been reported as an effective agent enhancing the biodegradation of hydrocarbons. However efficient, the surfactant's role in different processes that together enable the satisfying biodegradation should be thoroughly analysed and verified. In this research, we present the interactions of Triton X-100 wi...

Overall protein release greater than 75% in less than 1 h can be attained by exposing exponentially growing Escherichia coli cells to 0.4 M guanidine plus 0.5% Triton X-100 at 37 degrees C in medium. Cell growth stops immediately upon addition of the chemicals, but the cells are not lysed. Guanidine concentrations lower than 0.2 M, in conjunction with 0.5% Triton X-100, do not release significa...