نتایج جستجو برای: trypan blue staining
تعداد نتایج: 143116 فیلتر نتایج به سال:
A method for simultaneously detecting membrane permeability (characteristic of necrosis) and DNA fragmentation (characteristic of apoptosis) is described. By combining a common dye-exclusion method (Trypan Blue) with a commercially available terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) labeling kit, we have succeeded in developing a novel methodology for o...
Background & objectives: Pentavalent antimonials are the first-line drugs for treatment of leishmaniasis, which have multiple side effects such as drug toxicity. Moreover, parasite resistance to these drugs is rising around the world. Second-line drugs, including Amphotericin B and pantamidine have also side effects and expensive for patients. According to the cytotoxic effects of paraquat, thi...
PURPOSE Indocyanine green (ICG) and trypan blue have been advocated as vital stains for use during macular surgery. The safety of these agents was tested using a cell culture model. METHODS Human retinal pigment epithelium (RPE) and Müller cell lines were exposed to ICG over a range of concentrations up to 0.5%, and trypan blue up to 0.2%. Cells were exposed to each dye for 5, 15, or 30 minut...
background infertility is a serious social problem in advanced nations, with male factor in half of all cases of infertility. this study was conducted to determine the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster. methods twelve adult male hamsters were equally divided into azoospermic and control groups. busulfan was intraperitoneally used for i...
Aims: To report on the use of trypan blue (TB) 0.06% for staining the internal limiting membrane (ILM) and epiretinal membrane (ERM) during vitrectomy and report on their histology. Method: 14 consecutive patients with idiopathic macular hole or macular pucker (seven patients each) were prospectively recruited for ILM or ERM peel respectively. After pars plana vitrectomy and induction of poster...
Nitric oxide (NO) has signaling property in plants, therefore the effect of nitroprusside (SNP) as an NO producing agent on viability in the cell culture, lipid peroxidation and activity of superoxide dismutase, catalase and peroxidase in the callus of Catharantus roseus was investigated. Cell suspension was prepared from callus of Catharantus roseus, and the cells were treated with 0, 10, 50, ...
s of Annual Scientific Meeting of the Hong Kong Ophthalmological Society 2005 Clinical characteristics of intermittent exotropia in Hong Kong Flora HS Lau, C Yu, Dorothy Fan, Yip, Dennis SC Lam Hong Kong Eye Hospital Purpose: To describe the clinical characteristics of patients with intermittent exotropia at Hong Kong Eye Hospital. Methods: Prospective case series of 129 patients with diagnosis...
BACKGROUND BH3 mimetics are a class of drugs that antagonize the Bcl-2 family of apoptosis inhibitors. We have previously shown that these compounds can potentiate the activity of carboplatin against several ovarian cancer cell lines. However, recent clinical studies have highlighted that BH3 mimetics which antagonise Bcl-XL are associated with significant thrombocytopenia. This has led to the ...
Melanoma differentiation associated gene-7 (mda-7): a novel anti-tumor gene for cancer gene therapy.
BACKGROUND The mda-7 gene (melanoma differentiation associated gene-7) is a novel tumor suppressor gene. The anti-proliferative activity of MDA-7 has been previously reported. In this report, we analyze the anti-tumor efficacy of Ad-mda7 in a broad spectrum of cancer lines. MATERIALS AND METHODS Ad-mda7-transduced cancer or normal cell lines were assayed for cell proliferation (tritiated thym...
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