Trypsin inhibitors are studied in a variety of models for their anti-obesity and anti-inflammatory bioactive properties. Our group has previously demonstrated the satietogenic effect of tamarind seed trypsin inhibitors (TTI) in eutrophic mouse models and anti-inflammatory effects of other trypsin inhibitors. In this study, we evaluated TTI effect upon satiety, biochemical and inflammatory param...

The Kunitz-type trypsin inhibitor from soybean (STI) consists of 181 amino acid residues with two disulfide bridges. Its crystal structures have been determined in complex with porcine pancreatic trypsin in two crystal forms (an orthorhombic form at 1.75 A resolution and a tetragonal form at 1.9 A) and in the free state at 2.3 A resolution. They have been refined to crystallographic R-values of...

3-Formyl-4-hydroxyphenylguanidine hydrochloride and its Schiff base copper(II), zinc(II), and iron(III) chelates were synthesized and their inhibitory activity against bovine beta-trypsin were determined. Syntheses of Schiff base metal chelates were carried out from 3-formyl-4-hydroxyphenylguanidine, various L-amino acids, and divalent metal acetate. Their structures were established on the bas...

Inhibition of airway epithelial sodium channel (ENaC) function enhances mucociliary clearance (MCC). ENaC is positively regulated by channel-activating proteases (CAPs), and CAP inhibitors are therefore predicted to be beneficial in diseases associated with impaired MCC. The aims of the present study were to 1) identify low-molecular-weight inhibitors of airway CAPs and 2) to establish whether ...

Sodium metabisulfite (Na2S20S) was used to inactivate trypsin inhibitors in whole and cracked soybeans. Soybeans (400 g) steeped with 1600 ml aqueous O.IM Na2S20S at 30°C and 65°C for 2and 3-h were dehulled, dried, flaked and then defatted with either 6 batch extractions of hexane at ambient temperature or with supercritical CO2 for 15 min at 12,000 PSI and 84°C. The 2-h steep treatment at 65°C...

Kazal-type serine proteinase inhibitors are found in a large number of living organisms and play crucial roles in various biological and physiological processes. Although some Kazal-type serine protease inhibitors have been identified in leeches, none has been reported from Hirudinaria manillensis, which is a medically important leech. In this study, a novel Kazal-type trypsin inhibitor was iso...

Tryptases are trypsin-like serine proteinases found in the granules of mast cells. Although they show 40% sequence identity with trypsin and contain only 20 or 21 additional residues, tryptases display several unusual features. Unlike trypsin, the tryptases only make limited cleavages in a few proteins and are not inhibited by natural trypsin inhibitors, they form tetramers, bind heparin, and t...

In the various studies tha t have been done on the inhibition of proteolytic enzymes by serum, no distinction has been made in the inhibition of different enzymes. I t has generally been assumed that all proteolytic inhibition is caused by the same substance in serum and that this substance is occasionally increased in association with disease. There has been no evidence for the existence of se...

Currently, searching for new protease inhibitors is of interest to many scientists around the world because they play an important role in controlling harmful proteases. As terrestrial bioactive resources are becoming increasingly depleted, studies aimed at finding other sources, example, ocean. Some recent reports indicate that sponge- associated microbes produce substances with high biologica...

Bowman-Birk inhibitors (BBIs) are cysteine-rich and highly cross-linked small proteins that function as specific pseudosubstrates for digestive proteinases. They typically display a "double-headed" structure containing an independent proteinase-binding loop that can bind and inhibit trypsin, chymotrypsin and elastase. In the present study, we used computational biology to study the structural c...