Extravillous trophoblast (EVT) migration and invasion through the decidualized endometrium is essential to successful placentation. SGHPL-4 cells, an EVT cell line derived from first trimester placenta, is a widely used model of cytotrophoblast differentiation into an invasive phenotype. Here we describe a quantitative cell migration assay that can be modified to also measure cell invasion. SGH...

Twenty-two patients with rheumatoid arthritis and moderate to severe bilateral keratoconjunctivitis sicca were treated with artificial tears and a soluble slow-release artificial tear insert in a crossover trial. While using the inserts all but two patients reported an improvement in their symptoms. All the patients showed an improvement in the degree of conjunctival and corneal staining while ...

Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have developed a low copy number cosmid vector based on the E. coli F factor replicon (Fosmid). We have tested relative stability of human DNA inserts in Fosmids and in two conventional multicopy vectors (Lawr...

The modern orthopaedic implants for applications in hips, knees, shoulders, and spines are composed of hard metal alloys or ceramics a tribological sub-component that is made soft materials, with good frictional properties—e.g., UHMWPE (Ultra High Molecule Weight Polyethylene). need to be machined into their final shape after the polymerization consolidation blank profile near net shaped implan...

Abstract: Background: Medication package inserts (MPIs) provides patients with appropriate information on the rational use of drugs. This study evaluated level conformity MPIs regulatory drug labelling requirements and determined readability selected prescription MPIs. Methods: One hundred fifty-six commonly used prescribed antilipidemics, anticonvulsants, anti-diabetes, antiarthritis antihyper...