The total blood lymphocyte proliferation assay (TLP) was evaluated as a screening test for infection with Mycobacterium tuberculosis and was compared with the tuberculin (Mantoux) skin test. The results of TLP assays performed on 33 patients with tuberculosis and 37 non-tuberculous subjects were compared with results of skin tests performed in the previous year. There was a high correlation bet...

Methods are described for the evaluation in vitro of the effect of tuberculin on the leucocytes of peripheral blood. Washed leucocytes from a tuberculin sensitive host suspended in normal plasma are not lysed by tuberculin until after several hours of contact. Washed leucocytes from a tuberculin-sensitive host slowly release into normal plasma a factor which will cause the lysis of normal leuco...

Bovine tuberculosis is a chronic bacterial disease of cattle caused by Mycobacterium bovis. In many countries, bovine tuberculosis is a major infectious disease of cattle, other domesticated animals and certain wildlife populations. It has a public health significance. Bovine tuberculosis is usually diagnosed in the live animals based on the basis of delayed hypersensitivity reactions using var...

background with respect to the prevalence of tuberculosis in our country, bcg vaccination at birth seems to be an effective means of prevention. the present study was designed to evaluate the efficiency of bcg vaccination and factors influencing the tuberculin skin reaction. materials and methods the inoculum of live bcg organisms in vaccinated infants was evaluated at birth and the correlation...

Tuberculosis (TB) is the second most common contagious disease, caused by Mycobacterium tuberculosis (M. tuberculosis). Besides a rare laryngeal tuberculosis, pulmonary tuberculosis is the only one contagious form of the disease, although it can affect any organ of the human body. TB represents a new challenge to the doctors because it appears with numerous chronic diseases, affects immunocompr...

A prospective, double blind study was conducted to determine the degree of concordance of pen and palpation methods of measuring skin induration to Mantoux tuberculin tests. One hundred thirty-five skin tests were performed in patients with previously bacteriologically proved tuberculous disease. An experienced reader who was only able to see the forearm of the patient measured the induration b...

The method used to produce "Connaught" tuberculin purified protein derivative (PPD) is described. The tuberculin PPD for the multiple-puncture method was shown to be stable for at least 24 months at 5 C; tuberculin PPD for the intracutaneous method was shown to be stable at 5 C and 24 C for a period of 18 months in the presence of Tween 80. Evans blue or brillant vital red was added to tubercul...