Enzymatic discoloration of the diazo dye, Congo red (CR), by immobilized plant peroxidase from turnip "Brassica rapa" is investigated. Partially purified turnip peroxidase (TP) was immobilized by entrapment in spherical particles of calcium alginate and was assayed for the discoloration of aqueous CR solution. Experimental data revealed that pH, reaction time, temperature, colorant, and H2O2 co...

Various species and varieties of brassicas were evaluated for their potential to extend the grazing season and provide forage through late-fall and early winter in Colorado. The species and varieties evaluated included: rapes (Bonar, Winfred, and Barnopoli), turnips (Appin, Barkant, and Purple Top), kale (Kestral), turnip x rape hybrid (Pasja), swede (Major Plus), and radish (Groundhog). Each s...

Artificial microRNAs (amiRNAs) are the expression products of engineered microRNA (miRNA) genes that efficiently and specifically downregulate RNAs that contain complementary sequences. Transgenic plants expressing high levels of one or more amiRNAs targeting particular sequences in the genomes of some RNA viruses have shown specific resistance to the corresponding virus. This is the case of th...

The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....

Abstract Two near complete polerovirus genomes were assembled using high throughput sequencing (HTS) data from two separate samples of garlic cultivar ‘Glenlarge’ grown in Gatton, Queensland, Australia. Whole genome sequence comparisons showed that one contig shared 96.7% nucleotide identity with phasey bean mild yellows virus (MT966032.1) and the other, 99.8% turnip (MT586581.1). Phylogenetic ...

A total of 27 bacterial strains were isolated from apple, potato, turnip, onion, cucumber, zinger, lemon, spinach, radish and mint marketted in Labore, Pakistan. Highest frequency of occurrence (4%) was recorded for each of Enterobacter aggglomerans, Ensifer adhaerens and Bordetella pertussis, Brassica rapa and Kurthia gibsonii showed 30% frequency of occurrence. Frequency of all other bacteria...