Tween 80 (0.1%, v/v) added to Thermomonospora curvata growing in minimal medium caused a transient lowering of the dry cell mass, decreased the optimal growth temperature of the thermophile from 62 to 54°C, and increased extracellular esterase activity. Cells grown in the presence of Tween 80 had decreased concentrations of branched chain fatty acids and increased concentrations of oleic acid. ...

Purified tissue enzyme preparations, particularly bovine pancreatic deoxyribonuclease, have been shown to inhibit specifically pathogenic strains of Mycobacterium tuberculosis. Destruction of the enzymic activity did not influence this effect. Inhibition in Dubos’s medium was markedly increased by Tween 80 but not by other surface active agents. Antibiotic potentiation by Tween 80 alone or comb...

Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance reve...

Neonatal female rats were injected ip (0.1 ml/rat) with Tween 80 in 1, 5 or 10% aqueous solution on days 4-7 after birth. Treatment with Tween 80 accelerated maturation, prolonged the oestrus cycle, and induced persistent vaginal oestrus. The relative weight of the uterus and ovaries was decreased relative to the untreated controls. Squamous cell metaplasia of the epithelial lining of the uteru...

Injection of 0.1% saline solution of Tween 80 i.p. (3 X 3 cm3/kg/12 h) in the cat decreased the carbachol-induced growling response 36 h after the last injection. The content of 5-hydroxyindoleacetic acid (5-HIAA) increased in the hypothalamus, midbrain and amygdala, which indicates the increase in serotonin (5-HT) turnover and the 5-HT system activity. The results indicate that Tween 80 causes...

Under appropriate conditions, oleic acid replaces biotin for several lactic acid bacteria.‘, z This growth-promoting action of oleic acid is not nullified by excess avidin for Streptococcus jaecalis R2 or for several other organisms (Table I). Egg white did, however, block utilization of oleic acid by Lactobacillus arabinosus (Table I). This result is obtained whether oleic acid is added alone,...

The aim of the present study was to evaluate the stability of celecoxib (CXB) using novel nanoemulsion formulation. Optimized nanoemulsion formulation of CXB was prepared by low energy emulsification method. Stability studies were performed for the period of 3 months. Droplet size, viscosity and refractive index (RI) were determined during storage. Shelf life of nanoemulsion formulation was als...