S protein, a plasma glycoprotein with Mr 78,000, has been shown to interfere with the heparin-catalysed inhibition of thrombin by antithrombin III. This interaction was further evaluated in the present study. Native human blood was replaced by either radiolabelled antithrombin III or radiolabelled prothrombin in the reaction mixture, which was incubated at 37 degrees C. At various time interval...

In this report, we describe methods used to analyze the protein composition of sectioned frozen prostatic tissues by two-dimensional gel electrophoresis. Our results show a high degree of homology in two-dimensional electrophoretograms of proteins extracted from frozen sections of malignant prostate glands. Such homology was not apparent in protein patterns of benign hypertrophic prostatic tiss...

Proteomics is a promising new tool for functional genomics. In addition to two-dimensional gel electrophoresis, other methods that are based on liquid chromatography and mass spectrometry are now available to study proteins. In this brief article, we review the strengths and limitations of the proteomic approaches currently available to the researcher, and we provide examples of how proteomics ...

Biological function is mainly carried out by a dynamic population of proteins and peptides which may be used as markers for disease diagnosis, prognosis and as a guide for effective treatment. The study of proteins is called proteomics and it is generally performed by two-dimensional gel electrophoresis and mass spectrometric methods. However, gel-based proteomics is methodologically restricted...

Joan-J Bech-Serra1, Núria Colomé1, ProteoRed2, Andy Borthwick3, Martin Wells3, Juan-Pablo Albar4, Francesc Canals1 1 Proteomics Laboratory Medical Oncology Research Program. Vall d’Hebron University Hospital Research Institute, Barcelona, Spain; 2ProteoRed consortium. Spanish National Institute of Proteomics; 3Nonlinear Dynamics, Newcastle, UK; 4Centro Nacional de Biotecnologia-CSIC, Madrid, Spain

Canine factor VIII (FVIII) preparations isolated from cryoprecipitates by gel chromatography were pooled to provide one batch of antigen for simultaneous immunization of two rabbits and a goat. The goat and rabbit antisera had similar FVIII-neutralizing titers, but the latter had seven to ten times more precipitating titer for FVIII-related antigen (FVIII-RA). Absorption with material low in FV...

We evaluate renormalization factors of the domain-wall fermion system with various improved gauge actions at one loop level. The renormalization factors are calculated for quark wave function, quark mass, bilinear quark operators, threeand four-quark operators in MS scheme with the dimensional reduction(DRED) as well as the naive dimensional regularization(NDR). We also present detailed results...