Overexpression of the proinflammatory cytokine macrophage migration inhibitory factor (MIF) is linked to a number of autoimmune diseases and cancer. MIF production has been correlated to the number of CATT repeats in a microsatellite region upstream of the MIF gene. We have characterized the interaction of pituitary-specific positive transcription factor 1 (Pit-1) with a portion of the MIF prom...

Plant water transport occurs through interconnected xylem conduits that are separated by partially digested regions in the cell wall known as pit membranes. These structures have a dual function. Their porous construction facilitates water movement between conduits while limiting the spread of air that may enter the conduits and render them dysfunctional during a drought. Pit membranes have bee...

We characterized pit and mound (PM) topography resulting from catastrophic wind in the Coweeta Basin, and located 48 PMs a across a variety of forest types. Our measurements included pit length, width, and depth; and mound height, thickness, and width. Species of fallen trees were identified, and DBH (diameter at breast height, 1.37 m) was measured for biomass determination. We identified five ...

In the surface mining of mineral deposits, land resources suitable for agricultural purposes are inappropriately spent in large volumes. When deep open pits, overburden rocks mainly transported to surface. The optimal solution reducing area disturbed lands is placement internal dumps pit. This especially when a deposit with several pits where at least one them depleted. Therefore, it important ...

Aquatic field studies were conducted in Tha Mai District, Chanthaburi Province, Thailand. Larval habitats of Anopheles dirus were examined from November 1986 through June 1988 in 42 manmade gem pits. Larvae were found in pits containing clear water under full or partial shade. The abundance of different kinds of mosquito larvae were related to seasonal changes in these aquatic habitats. Variati...

To test the role of wtPIT-1 (PITWT) or PIT-1 (R271W) (PIT271) in somatolactotroph cells, we established, using inducible lentiviral vectors, sublines of GH4C1 somatotroph cells that allow the blockade of the expression of endogenous PIT-1 and/or the expression of PITWT or PIT271, a dominant negative mutant of PIT-1 responsible for Combined Pituitary Hormone Deficiency in patients. Blocking expr...

Nuclear extracts prepared from growth hormone-secreting (GC) and prolactin-secreting (235-1) rat anterior pituitary cell lines were compared for their ability to bind to the DNA sequences conferring tissue-specificity to the expression of the rat growth hormone (rGH) gene promoter. Cell-specific differences in the interaction of Pit-1, a tissue-specific member of the POU-domain transcription fa...