Two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of Sulfamethoxazole (SMX) and Phthalazine (PHZ) by High Performance Liquid Chromatography (HPLC). Multivariate calibration techniques such as Classical Least Squares (CLS) and Inverse Least Squares (ILS) were introduced into HPLC to determine the quantification by using UV detecto...

An accurate, reproducible and sensitive method for the quantitative determination of sarcophine in the organic extract of the Red Sea soft coral Sarcophyton species was developed and validated. Sarcophine concentration was determined by RP HPLC using ODS column. The mobile phase was made up of 70% acetonitrile in deionized water and the pH was adjusted to 3.5 with phosphoric acid. The flow rate...

We describe a "high-performance" liquid chromatographic (HPLC) method for accurately determining creatinine in serum. After prechromatographic precipitation of protein, we performed isocratic ion-exchange chromatography with ultraviolet detection (234 nm). Analytical results showed linearity up to 1770 mumol/L, a detection limit of 22 mumol/L, an average analytical recovery of 101%, and a CV ra...

A new intracavernous formulation to overcome the failure of standard treatments of erectile dysfunction after radical prostatectomy was developed. This formulation contains prostaglandin E1 (pGE1) at 15 μg/ml in association with papaverin and urapidil at 15 mg/ml and 2.5 mg/ml respectively. Since pGE1 was the main compound to be subject to physico-chemical degradation, its degradation and the c...

HPLC with various detection methods, including ultraviolet absorbance, is frequently used to separate and measure creatinine and creatine in serum, plasma, and urine. Current HPLC and other analytical methods for the measurement of creatinine, including capillary electrophoresis and gas chromatography–mass spectrometry, have been reviewed recently (1 ). In 1990, Paroni et al. (2 ) reported a ca...

Triacylglycerol (TAG) composition in vegetable oils was collaboratively analyzed in 9 laboratories using high-performance liquid chromatography (HPLC) equipped with a refractive index detector. Dococyl (C22) and triacontyl (C30) silica columns were used for analysis. The TAG molecular species in soybean, rapeseed, and palm oils were individually separated on the chromatogram. The collaborative ...

A simple reversed-phase chiral HPLC method has been developed and validated for direct separation of the enantiomers of zaltoprofen. Separation of the enantiomers was tested on numerous commercial chiral HPLC columns. Separation was best (resolution, RS = 3) on a Chiralcel OJ-RH stationary phase. Typical retention times of the S and R enantiomers were approximately 14 and 16 min, respectively. ...

Ergönül P.G., Nergiz C. (2010): Determination of organic acids in olive fruit by HPLC. Czech J. Food Sci., 28: 202–205. Organic acids (oxalic, citric, malic, and succinic) contents of Domat, Memecik and Uslu varieties of olives grown in Turkey were investigated using HPLC method. Organic acids were extracted from olives with water-methanol mixture solution 75:25 (v/v) and were analysed through ...

Solubility measurements using chemiluminescent nitrogen detection (CLND) has advantages of reduced compound requirement and increased throughput compared to UV-spectrophotometric and HPLC-based measurements. CLND with direct flow injection was evaluated for the measurement of thermodynamic solubility to support drug discovery. The limit of quantitation (LOQ), accuracy, and day-to-day reproducib...

Forced degradation studies are used to validate analytical procedures for the drug substance and products. of Pimecrolimus its cream were performed under stressed alkaline, acidic, oxidative thermal conditions according ICH guidelines Q1A(R2). A sensitive stability indicating RP-HPLC method was purposed validated separation Desmethyl Pimecrolimus. The chromatographic achieved with Phenomenax Lu...