Abstract The interest toward promoting walking culture has been increased dramatically especially in many cities across the nation. This is also included universities worldwide that have started seeking ways to increase pedestrian activities. Hence, campus planners must address mobility and accessibility needs of their communities ensure safety, functionality conducive living learning environme...

Adenylyl transferase (ATase) is the bifunctional effector enzyme in the nitrogen assimilation cascade that controls the activity of glutamine synthetase (GS) in Escherichia coli. This study addresses the question of whether the two antagonistic activities of ATase (adenylylation and deadenylylation) occur at the same or at different active sites. The 945 amino acid residue ATase has been trunca...

Phosphoglycosyltransferases (PGTs) are families of integral membrane proteins with intriguingly diverse architectures. These enzymes function to initiate many important biosynthetic pathways including those leading to peptidoglycan, N-linked glycoproteins and lipopolysaccharide O-antigen. In spite of tremendous efforts, characterization of these enzymes remains a challenge not only due to the i...

Using the method of genomic binding-site cloning, we identified three target genes of the developmental regulator, the product of priB gene (PRIB) in Lentinula edodes: the previously cloned priB and uck1 (UMP-CMP kinase gene) and a new gene, which we named mfbC. Identification of the former two genes was expected, because the promoter regions of priB and the gene encoding UMP-CMP kinase (uck1) ...

Practical enzyme-based routes for the syntheses of uridine 5'-diphosphogalactose (UDP-Gal) and uridine 5'-diphospho-N-acetylgalactosamine (UDP-GalNAc) on millimole scales have been developed. The activity of galactokinase (EC 2.7.L.6) in crude enzyme extracts from galactose-adapted yeast, coupled to a regenerating system for ATP, provides convenient and economical access to galactose-a-1-phosph...

Acivicin (NSC 163501) and dichloroallyl lawsone (NSC 126771) are potent inhibitors of nucleotide biosynthesis with consequent anti-cancer activity against certain experimental tumors. To determine in detail the metabolic events induced by each inhibitor, we have devised a new two-dimensional chromatographic procedure for measurement of the concentrations of all pyrimidine intermediates and some...

The incorporation of bicarbonate-14C into pyrimidines of the Xovikoff aceites tumor cell has been studied. It was shown that bicarbonate was incorporated into carbamylaspartate and into the C-2 of uracil nucleotides. The incorporation was increased by both glutamine or ammonia; glutamine was the more effective nitrogen donor. Exogenous orotic acid inhibited the formation of undine monophosphate...

Although several languages have been proposed for dealing with uncertainty in the Semantic Web (SW), almost no support has been given to ontological engineers on how to create such probabilistic ontologies (PO). This task of modeling POs has proven to be extremely difficult and hard to replicate. This paper presents the first tool in the world to implement a process which guides users in modeli...

We propose a new classification of thyroid follicular cell tumors which is correlated with patient's prognosis. It is unique as to two new categories: borderline malignancy between benign and malignant, and moderately differentiated adenocarcinoma (MDA) as a differentiation classification to stratify tumor aggressiveness. As to diagnostic criteria, we recommend invasiveness (capsular and vasc...