Synthetic genes encoding single-chain variable fragments (scFvs) of NC10 anti-neuraminidase antibody were constructed by joining the V(L) and V(H) domains with linkers of fifteen, five, four, three, two, one and zero residues. These V(L)-V(H) constructs were expressed in Escherichia coli and the resulting proteins were characterized and compared with the previously characterized NC10 scFv prote...

List homomorphisms generalize list colourings in the following way: Given graphs G; H , and lists L(v) V (H); v 2 V (G), a list homomorphism of G to H with respect to the lists L is a mapping f : V (G) ! V (H) such that uv 2 E (G) implies f (u)f(v) 2 E (H), and f (v) 2 L(v) for all v 2 V (G). The list homomorphism problem for a xed graph H asks whether or not an input graph G together with list...

Figure S1. Cont. 68 PnLac1 95 PoLac 95 PsLac 95 PpLac 95 LsLac 65 HmLac . M M M M . . V V V V . . L L L L . . S S S S . . T T T T . . K K K K . . L L L L . . A A V V . . A A A A . . L L L L . . V V V V . . A A A A . . S S S S . . L L L L . . P P P P . . F F F F . . V V V V . . L L F F . . A A A A . . A A V V . . T T T T . . K K K K . . K K K K . . L L L L . . D D D D . . F F F F . . H H H H . ....

the emph{harary index} $h(g)$ of a connected graph $g$ is defined as $h(g)=sum_{u,vin v(g)}frac{1}{d_g(u,v)}$ where $d_g(u,v)$ is the distance between vertices $u$ and $v$ of $g$. the steiner distance in a graph, introduced by chartrand et al. in 1989, is a natural generalization of the concept of classical graph distance. for a connected graph $g$ of order at least $2$ and $ssubseteq v(g)$, th...

h-point standard addition method (hpsam) was applied for the simultaneous determination of v(v) and fe(iii). the method is based on the complex formation of v(v) and fe(iii) with chromazurol s (cas) in acidic media and in the presence of cetyltrimethylammonium bromide (ctab) as a micellizing agent. acidic media and the presence of a micellar system improve selectivity and sensitivity. fe (iii) ...

For digraphs D and H , a mapping f : V (D)→V (H) is a homomorphism of D to H if uv ∈ A(D) implies f (u) f (v) ∈ A(H). If, moreover, each vertex u ∈ V (D) is associated with costs ci (u), i ∈ V (H), then the cost of the homomorphism f is ∑ u∈V (D) c f (u)(u). For each fixed digraph H , we have the minimum cost homomorphism problem for H (abbreviated MinHOM(H )). The problem is to decide, for an ...

The topology of an interconnection network can be modeled by a graph G=(V(G),E(G)). connectivity G is parameter used to measure the reliability corresponding network. direct product important product. This paper mainly focuses on super connectedness graphs and cycles. G, denoted κ(G), size minimum vertex set S⊆V(G) such that G−S not connected or has only one vertex. said connected, simply super...

Suppose G is a graph and H is a subgraph of G. Let L be a mapping that assigns to each vertex v of G a set L(v) of positive integers. We say (G,H) is backbone L-colourable if there is a proper vertex colouring c of G such that c(v) ∈ L(v) for all v ∈ V , and |c(u) − c(v)| > 2 for every edge uv in H . We say (G,H) is backbone k-choosable if (G,H) is backbone Lcolourable for any list assignment L...

NS5B is pivotal RNA dependent RNA polymerase (RdRp) of HCV and NS5B function interfering halts the virus infective cycle. This work aimed to produce cell penetrable humanized single domain antibodies (SdAb; VH/V(H)H) that interfere with the RdRp activity. Recombinant NS5BΔ55 of genotype 3a HCV with de novo RNA synthetic activity was produced and used in phage biopanning for selecting phage clon...