نتایج جستجو برای: vhh nanobody

تعداد نتایج: 1067  

Journal: :International Journal of Molecular Sciences 2018

Journal: :Applied and environmental microbiology 2011
M Cruz Martín Neha Pant Victor Ladero Gökçe Günaydin Kasper Krogh Andersen Beatriz Alvarez Noelia Martínez Miguel A Alvarez Lennart Hammarström Harold Marcotte

A series of expression cassettes which mediate secretion or surface display of antibody fragments was stably integrated in the chromosome of Lactobacillus paracasei. L. paracasei producing surface-anchored variable domain of llama heavy chain (VHH) (ARP1) directed against rotavirus showed efficient binding to rotavirus and protection in the mouse model of rotavirus infection.

Journal: :Inflammation 2021

The currently used anti-cytokine therapeutic antibodies cannot selectively neutralize pathogenic cytokine signalling that cause collateral damage to protective cascades. single domain chain firstly discovered in Camelidae displays fully functional ability antigen-binding against variable targets, which has been seemed as attractive candidates for the next-generation biologic drug study. In this...

Background: Diarrhea caused by Escherichia coli is a major cause of morbidity and mortality in young animals. Few treatment options are available, mainly antibiotic therapy increasingly limited by resistance to commonly used drugs.Objectives: The aim of this work was to develop immunotherapy based on the use of camel VHH antibody fragments, or nanobodies,...

Journal: :Molecular cancer therapeutics 2008
Bernard M Tijink Toon Laeremans Marianne Budde Marijke Stigter-van Walsum Torsten Dreier Hans J de Haard C René Leemans Guus A M S van Dongen

The approximately 15-kDa variable domains of camelid heavy-chain-only antibodies (called Nanobodies) can easily be formatted as multivalent or multispecific single-chain proteins. Because of fast excretion, however, they are less suitable for therapy of cancer. In this study, we aimed for improved tumor targeting of a bivalent anti-epidermal growth factor receptor (EGFR) Nanobody (alphaEGFR-alp...

2012
Maria J.W.D. Vosjan Jo Vercammen Joost A. Kolkman Marijke Stigter-van Walsum Hilde Revets

Hepatocyte growth factor (HGF) and its receptor c-Met are associated with increased aggressiveness of tumors and poor prognostic outcome of patients with cancer. Here, we report the development and characterization of therapeutic anti-HGF (aHGF)-Nanobodies and their potential for positron emission tomographic (PET) imaging to assess HGF expression in vivo. Two aHGF-Nanobodies designated 1E2 and...

2011
Chueh-Ling Kuo George A. Oyler Charles B. Shoemaker

Botulinum neurotoxin (BoNT), a Category A biodefense agent, delivers a protease to motor neuron cytosol that cleaves one or more soluble NSF attachment protein receptors (SNARE) proteins involved in neurotransmission to cause a flaccid paralysis. No antidotes exist to reverse symptoms of BoNT intoxication so severely affected patients require artificial respiration with prolonged intensive care...

2016
Xinyang Li Xiaobo Duan Kai Yang Wei Zhang Changjiang Zhang Longfei Fu Zhe Ren Changxi Wang Jinghua Wu Ruxue Lu Yanrui Ye Mengying He Chao Nie Naibo Yang Jian Wang Huanming Yang Xiao Liu Wen Tan

Compared to classical antibodies, camel heavy chain antibodies (HCAbs) are smaller in size due to lack of the light chain and the first constant domain of the heavy chain (CH1 region). The variable regions of HCAbs (VHHs) are more soluble and stable than that of conventional antibodies (VHs). Even with such simple structure, they are still functional in antigen binding. Although HCAbs have been...

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