Pseudomonas aeruginosa strain PA14 is an opportunistic human pathogen capable of infecting a wide range of organisms including the nematode Caenorhabditis elegans. We used a non-redundant transposon mutant library consisting of 5,850 clones corresponding to 75% of the total and approximately 80% of the non-essential PA14 ORFs to carry out a genome-wide screen for attenuation of PA14 virulence i...

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for the identification of reliable determinants of virulence. In this review, we focus on identified Candida albicans genes whose deletant strains have been tested in experimental virulence assays. We d...

In fish, bacteriosis has been widely linked with Aeromonas species, but co-infection by these bacteria little addressed. The aims of this study were to report on an outbreak disease in pirarucu Arapaima gigas caused and investigate experimental characterize resistance profile, virulence factors phenotypic molecular differentiation. Fish samples clinical signs collected used co-infection. bacter...

A custom-designed microarray containing 220 virulence genes of Streptococcus pyogenes (group A Streptococcus [GAS]) was used to test group C Streptococcus dysgalactiae subsp. dysgalactiae (GCS) field strains causing bovine mastitis and group C or group G Streptococcus dysgalactiae subsp. equisimilis (GCS/GGS) isolates from human infections, with the latter being used for comparative purposes, f...

We previously reported that disruption of the yjbI gene reduced virulence Staphylococcus aureus. In this study, we found in both silkworms and mice was restored by introducing yjbH but not to genes-disrupted mutants, suggesting yjbH, downstream a two-gene operon-yjbIH, is responsible for phenomenon. further observed decrease various surface-associated proteins changes cell envelope glycostructu...

Background: Campylobacter is recognized as a major cause of foodborne gastroenteritis in humans in many countries and may be transferred from animals to humans. The consumption of chicken meat is identified as a major cause of Campylobacter infection in humans. Aims: To find out the contamination rate of chicken meat with Campylobacter</em...

Staphylococcus felis is one of staphylococci residing on skin cats, and has been increasingly reported as a potential facultative pathogen. A recently isolated S. (named HARRANVET strain) from necrotizing fasciitis case cat was characterized using whole genome sequencing (WGS), assembled in silico screened for putative virulence antimicrobial resistance genes. The isolate pan-susceptible to all...

Escherichia coli O103, harbored in the hindgut and shed in the feces of cattle, can be enterohemorrhagic (EHEC), enteropathogenic (EPEC), or putative non-pathotype. The genetic diversity particularly that of virulence gene profiles within O103 serogroup is likely to be broad, considering the wide range in severity of illness. However, virulence descriptions of the E. coli O103 strains isolated ...

The present work was conducted to compare the occurrence of Escherichia coli possessing virulence and ESBL genes in backyard and farmed poultry. Three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from West Bengal, India. Among the E. coli isolates of backyard poultry (O2, O10, O25, O55, O60, O106, UT), none of them posse...

We characterized 144 Escherichia coli isolates from severe cellulitis lesions in broiler chickens from South Brazil. Analysis of susceptibility to 15 antimicrobials revealed frequencies of resistance of less than 30% for most antimicrobials except tetracycline (70%) and sulphonamides (60%). The genotyping of 34 virulence-associated genes revealed that all the isolates harbored virulence factors...