Objective(s): Severe injuries are often associated with tissue hypothyroidism, elevated damaging mediators in circulation, and broken gut epithelial barrier.  However, the relationships between the hypothyroid state and gut epithelial damage are largely unknown.  Therefore, in this study, we investigated the effects of L-thyroxine (T4) on in vitro models of intact and ...

Gut mucosal mast cells (MMC), which are nearly absent in normal mice are abundant during nematode infection. In normal mice, study of MMC precursors (MMC-P: cells giving rise to MMC colonies in the presence of IL-3) show that: (a) their frequency, judged by limiting dilution is very high in bone marrow (BM) and gut, and very low in most lymphoid organs and thoracic duct lymph (TDL); (b) gut MMC...

The capacity of cell-free preparations of rat small intestinal mucosa to synthesize and release lipoproteins was investigated. Palmitate-l-l% was administered intragastrically and 30 min later labeled cell-free fractions from homogenates of intestinal mucosa were prepared. The fractions (whole homogenate, mitochondria, and microsomes) were incubated in forttied media containing 3H-labeled amino...

The capacity of cell-free preparations of rat small intestinal mucosa to synthesize and release lipoproteins was investigated. Palmitate-l-l% was administered intragastrically and 30 min later labeled cell-free fractions from homogenates of intestinal mucosa were prepared. The fractions (whole homogenate, mitochondria, and microsomes) were incubated in forttied media containing 3H-labeled amino...

The effect of different L-phenylalanine (Phe) concentrations (0.12-12.1 mM) on acetylcholinesterase (AChE), (Na+,K+)-ATPase and Mg2+-ATPase activities was investigated in homogenates of adult rat whole brain and frontal cortex at 37 degrees C. AChE, (Na+,K+)-ATPase and Mg2+-ATPase activities were determined after preincubation with Phe. AChE activity in both tissues showed a decrease up to 18% ...

We evaluated the cathepsin A activity of a parietal thrombus of an abdominal aortic aneurysm. We compared this activity to that of a retracted blood clot homogenate. Cathepsin A of aneurysm parietal thrombus homogenate and blood clot homogenate showed the highest activity on Z-Phe-Ala. It was lower on Z-Phe-Phe, Z-Glu-Tyr, Z-Glu-Phe, Z-Gly-Phe, and the lowest activity was on Z-Gly-Ala. We concl...

There is very limited knowledge about possible pharmacokinetic interactions between opioid analgesics and nonsteroidal antiinflammatory drugs (NSAIDs), which are commonly used in combination for the treatment of chronic pain. The major metabolic pathway of the weak opioid codeine is glucuronidation to codeine6-glucuronide. Therefore we investigated the influence of the NSAID diclofenac on the f...

In vitro propagation of Cavendish banana is an alternative to produce healthy, uniform and sufficient plant materials high qualities. Appropriate culture media formulation needed for shoot growth rooting stage. This experiment aimed study effects IAA organic supplements on in rooting. Explants were subjected MS medium with various supplements, a factorial arrangement completely randomized desig...

The “homogenate technique” is at present widely used for the study of enzymatic reactions in various types of biological material. In the original technique (1)’ an attempt n-as made to eliminat,e cell structure and to disperse intracellular constitucnt,s so widely that endogenous respiration was eliminated by dilution, while particular phases of metabolism could be restored by the addition of ...

The factors controlling the initiation, maintenance, and ces sation of hepatic cell mitosis in response to partial hepatectomy are not completely understood. Evidence offered by parabiont studies favors considering the presence of a humoral factor (7, 10, 16). The present study demonstrates the effect of the postulated humoral factor upon tumor cells present in the liver during the 12-day perio...