OBJECTIVE To develop an in vitro strategy to support the growth of early-stage follicles and produce mature oocytes competent for fertilization. DESIGN Whole ovaries from 8-day-old mice were cultured for 4 days, and then secondary follicles were isolated and cultured for 12 days in a three-dimensional alginate or fibrin-alginate (FA) hydrogel matrix. SETTING University-affiliated laboratory...

Holstein cow ovaries obtained at a slaughterhouse were used to study the influence of the oocyte collection methods (slicing, puncture, aspiration I and II) on recovery efficiency and subsequent in vitro maturation and embryonic development competence of immature oocytes recovered. In the slicing method, the whole ovarian was chopped into small pieces with a surgical blade. In the puncture meth...

PURPOSE As with many cancers thought to be of epithelial origin, expression profiling studies of ovarian cancer have relied on a variety of sources of normal cells for comparison with tumors, including whole ovary samples (WO), ovarian surface epithelium (OSE) exposed to short-term culture, and immortalized OSE cell lines (IOSE). Our purpose was to assess the impact of the use of different type...

CCNE1 gene amplification is present in 15-20% ovary tumor specimens. Here, we showed that Cyclin E1 (CCNE1) was overexpressed in 30% of established ovarian cancer cell lines. We also showed that CCNE1 was stained positive in over 40% of primary ovary tumor specimens regardless of their histological types while CCNE1 staining was either negative or low in normal ovary and benign ovary tumor tiss...

objective(s): several researchers have reported the relationship between infertility in male and varicocele for so many years but the implication of varicocele in female patients is remains elusive. here, we aim to examine the effects of unilateral varicose ovarian vein on antioxidant capacity and oocyte quality of rat ovary after the experimental creation of varicocele in female rats. material...

background: peritoneal tuberculosis accounts 1-2% of all forms of tuberculosis. peritoneal tuberculosis is an important differential diagnosis for ovarian cancer in women with ascites, adnexal mass and elevated cancer antigen 125 (ca125) levels. we report a case of a 32- year -old woman with multiple miliary peritoneal deposits mimicking advanced ovarian carcinoma. case presentation: a 32-year-...

background: retinoids have been suggested to play a role in oogenesis and oocyte survival. objective: in the present study the effects of retinol palmitate were investigated on differential follicular counts in response to superovulation as well as follicle quality after vitrification of ovaries. materials and methods: ten, 4 week old female balb/c mice were randomly assigned to either paraffin...

background: some studies propose that changes in leptin concentrations (above or under the normal range) result in infertility. therefore, we investigated serum and follicular fluid leptin concentrations in infertile women with polycystic ovary syndrome (pcos). objective: to study serum and follicular fluid leptin concentrations in infertile women with pcos. materials and methods: we conducted ...

while ovarian masses are common findings in gynecology, unusually huge masses are quite rare. however, such cases can still be found today. mismanagement of common complaints in patients can be a leading cause of facing such findings. herein, we introduce a case of a giant ovarian cyst, which was mismanaged in a young virgin female patient. the patient had received gastrointestinal medications ...