Increased expression of cyclooxygenase (COX) 2 and the production of PGs appear to provide a survival advantage to transformed cells through the inhibition of apoptosis, increased attachment to extracellular matrix, increased invasiveness and the stimulation of angiogenesis. The purpose of this study was to determine whether an angiogenic antagonist, SU5416, could inhibit endogenous and phorbol...

Bronchial asthma is developed as an immune response to allergen challenges accompanied by inflammation and fibrosis implicated in airway remodeling. To reveal the causative implication of Cu-containing amino oxidases semicarbazide-sensitive amine oxidase (SSAO), DAO lysyl (LOX) BA development we used their irreversible inhibitor semicarbazide guinea pig model induced ovalbumin. Semicarbazide wa...

oxidase 3 . Moreover, methylene blue was shown to be a strong inhibitor of the overall reaction. Oneelectron acceptors are also ineffective with the Dspecific enzyme; however, methylene blue and 2.6dichlorophenol-indophenol are able to reoxidize the reduced D-6-hydroxynicotine oxidase. In the presence of these dyes the rates of the overall process are higher than those with oxygen. Anaerobic ti...

Despite the important role of pyrazinamide in tuberculosis treatment, little is known about the mechanism of pyrazinamide-induced hepatotoxicity. We inhibited xanthine oxidase in HepG2 cells by using a nontoxic concentration of allopurinol, a well-known xanthine-oxidase inhibitor. This increased in vitro pyrazinamide toxicity in HepG2 cells, which suggests that the hydroxy metabolites of pyrazi...

This study used integrated omics technologies to investigate the potential novel pathways and enzymes for 1,4-dioxane degradation by a consortium enriched from activated sludge of domestic wastewater treatment plant. An unclassified genus belonging Xanthobacteraceae increased significantly after magnetic nanoparticle-mediated isolation degraders. Species with relatively higher abundance (> 0.3%...

abstract background and objective: highly active antiretroviral therapy (haart) can effectively prevent the progression of hiv-1 replication and increase life expectancy. there are numerous causes of treatment failure and the leading one is drug resistance. thus, we aimed to determine the hiv rt gene drug resistance mutations in patients treated with antiretroviral medications. material and met...

We recently reported that NADH oxidase is one of the major enzymes responsible for superoxide (O(2)(-)*) production in the rat kidney. However, the functional significance of NADH oxidase-mediated O. production and the mechanisms regulating this enzyme activity are poorly understood. Using fluorescence microscopic imaging analysis, the present study demonstrated that thick ascending limbs of He...

We previously reported that the all-cis isomer of arachidonic acid, the most naturally occurring isoform of this fatty acid, reduced cuprous copper ion-induced conversion of xanthine dehydrogenase into its reactive oxygen species generating form, xanthine oxidase. In the present study, the effects of all-trans isomer of arachidonic acid, in comparison with cis isomer of arachidonic acid, on the...

Hu, Taishan, Satish P. RamachandraRao, Senthuran Siva, Cathryn Valancius, Yanqing Zhu, Kalyankar Mahadev, Irene Toh, Barry J. Goldstein, Marilyn Woolkalis, and Kumar Sharma. Reactive oxygen species production via NADPH oxidase mediates TGF-induced cytoskeletal alterations in endothelial cells. Am J Physiol Renal Physiol 289: F816–F825, 2005; doi:10.1152/ajprenal.00024.2005.—Cytoskeletal alterat...

Mammalian polyamine catabolism is under the control of two enzymes, spermidine/spermine N1-acetyltransferase and the flavin adenine dinucleotide-dependent polyamine oxidase (PAO). In this study, the cloning and initial characterization of human PAO is reported. A 1894-bp cDNA with an open reading frame of 1668-bp codes for a protein of 555 amino acids. In vitro transcription/translation of this...