This is a prospective, descriptive and analytic study conducted from July 2011 to September 2011 at the Children National Hospital Albert Royer of Dakar and at the Vélingara Health District. It was focused on children under 15 without reference to HIV status. For each child, a sample of stool was examined by the Ziehl-Neelsen modified staining and by ELISA using the “Cryptosporidium Antigen Det...

BACKGROUND The main goal of the present study was to develop a new sensitive and specific PCR based method for Identification of Cryptosporidium sp. using novel primers from 18S ribosomal RNA. Cryptosporidiosis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. To the best of our knowledge this is the first study regarding develop a new PCR...

BACKGROUND Document seasonality occurrence and epidemiologic characteristics of Cyclospora cayetanensis infections during a 10-year period from patients consulting at the University Hospital, Honduras. METHODS Retrospective non interventional hospital-based study analyzed laboratory results from the period 2002 to 2011 of fresh and Ziehl-Nielsen carbolfuchsin stained routine stool samples rec...

The Ziehl–Neelsen (ZN) stain, also known as the acidfast stain, has been reported to be helpful in detection and identification of Schistosoma eggs. In histological sections, Schistosoma mansoni egg shells appear as ZN positive and Schistosoma haematobium shells as ZN negative. The staining target of the responsible ZN component (carbolfuchsin) in the shell is unknown. Because carbolfuchsin is ...

OBJECTIVE To determine the accuracy of Ziehl-Neelsen microscopy in the diagnosis of TB in setings of high HIV prevalence. DESIGN Cross-sectional descriptive study. SETTING Hospitals serving areas of high human immunodeficiency virus prevalence in western Kenya. The study was conducted between September 2007 and September 2009. RESULTS In total, 341/872(39.1%) of the TB suspects were posit...

Screening for tuberculosis in high-prevalence countries relies on sputum smear microscopy. We present a method for the automated identification of Mycobacterium tuberculosis in images of Ziehl-Neelsen-stained sputum smears obtained using a bright-field microscope. We use two stages of classification. The first comprises a one-class pixel classifier for object segmentation. Geometric transformat...

I n underdeveloped countries, laboratory faci l i ties for the bacterio­ logical d iagnosis of tubercu losis are, at present , very l imited . Cultural methods are un l i kely to be used on a large scale for many years to come. It is , therefore, important to investigate the most economical method of examin ing smears for tubercle baci l l i . Fluorescence microscopy was introduced by H agemann...

Background: Microbiological confirmation of tuberculous meningitis (TBM) remains problematic. We assessed the diagnostic performance of a modified Ziehl-Neelsen (MZN) staining method that showed promise in earlier studies. Methods: Patients evaluated for TBM in Shaanxi province, China, were prospectively enrolled from May, 2011 to April, 2013. Cerebrospinal fluid (CSF) specimens were evaluated ...