OBJECTIVES It is important to characterize the surface of dental amalgam in order to understand the process of mercury release from amalgam restorations in the oral cavity. The mercury evaporation occurs not only from the newly made restoration but also from the set material. METHODS The surfaces of four different types of amalgams, which had been well set, were analyzed with X-ray photoelect...

A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. T...

In order to exploit a fast-growing Paulownia hardwood as an energy crop, xylose-enriched hydrolysate was obtained in this work increase the ethanol concentration using hemicellulosic fraction, besides already widely studied cellulosic fraction. For that, elongata x fortunei submitted autohydrolysis treatment (210 °C or S0 of 4.08) for xylan solubilization, mainly xylooligosaccharides. Afterward...

Highly diastereoselective and enantioselective catalytic capture of chiral zinc enolates using nitroolefins as electrophiles is described. The tandem products γ-nitro ketones were obtained in good yields with high diastereoselectivities and enantioselectivities. The γ-nitro ketones were readily hydrogenated to the optically enriched and diastereomerically pure chiral pyrrolidines with four cont...

Two classes of metal ion binding sites in horse liver alcohol dehydrogenase are distinguished by the observed rates of replacement of zinc by cobalt in acetate and in Z-(N-morpholine)-ethane sulfonic acid buffers. Hybrid enzymes containing both zinc and cobalt metal ions have been prepared and exhibit visible absorption maxima at 655 nm and 740 nm. Binding of azide, a substrate-competitive inhi...

Copper, zinc, and iron were quantitated in the serum and lymphoid cells of the peripheral blood of healthy children and children with acute lymphocytic leukemia. Copper and iron con centrations in serum and cells were significantly higher and zinc concentration in the cells significantly lower in leukemic patients than in healthy donors, whereas the increase of zinc in the serum was not signifi...

In the yeast Saccharomyces cerevisiae, the mineral zinc is essential for growth and metabolism. Depletion of zinc from the growth medium of wild type cells results in changes in phospholipid metabolism, including an increase in phosphatidylinositol content (Iwanyshyn, W. M., Han, G.-S., and Carman, G. M. (2004) J. Biol. Chem. 279, 21976-21983). We examined the effects of zinc depletion on the r...

Copper, zinc, and iron were quantitated in the serum and lymphoid cells of the peripheral blood of healthy children and children with acute lymphocytic leukemia. Copper and iron concentrations in serum and cells were significantly higher and zinc concentration in the cells significantly lower in leukemic patients than in healthy donors, whereas the increase of zinc in the serum was not signific...

Selenium (Se) enriched yeast, produced by growing of Saccharomyces cerevisiae in Se rich media, is a recognized source of organic food-form of Se. Effects of culture conditions (temperature, fermentation time, initial pH value, shaking speed, as well as time and concentration of Se added to medium) on the bioaccumulation of Se in yeast were investigated by Plackett-Burman design in this paper. ...

BACKGROUND Traumatic brain injury (TBI) is one of the leading causes of disability and death among young people. Although much is already known about secondary brain damage the full range of brain tissue responses to TBI remains to be elucidated. A population of neurons located in cerebral areas associated with higher cognitive functions harbours a vesicular zinc pool co-localized with glutamat...