Genome engineering methods in E. coli allow for easy to perform manipulations of the chromosome in vivo with the assistance of the λ-Red recombinase system. These methods generally rely on the insertion of an antibiotic resistance cassette followed by removal of the same cassette, resulting in a two-step procedure for genomic manipulations. Here we describe a method and plasmid system that can ...

A lethal dose (1 Mrad) of gamma rays was given to wheat leaf tissue to restrict migration of the energy of red light photoexcitation to the photosynthetic pigment system. This dose of gamma radiation had no apparent effect on chloroplast ultrastructure in tissue that received no illumination. Subsequent illumination with red light, used to induce chlorophyll-sensitized photodestruction of ultra...

An asd-deleted (Deltaasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype ...

The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, λ(ex) = 552 nm, λ(em) = 578 nm) and its bright far-red fluorescent variant Katushka (λ(ex) = 588 nm, λ(em) = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have be...

By a combination of analytical and numerical techniques, we demonstrate the existence of spherical, asymptotically flat traversable wormholes supported by exotic matter whose stress tensor relative to the orthonormal frame of Killing observers takes the form of a perfect fluid possessing anisotropic pressures and subject to linear equations of state: τ = λρc, P = μρc. We show that there exists ...

The crystal structures of the far-red fluorescent proteins (FPs) eqFP650 (λ(ex)(max)/λ(em)(max) 592/650 nm) and eqFP670 (λ(ex)(max)/λ(em)(max) 605/670 nm), the successors of the far-red FP Katushka (λ(ex)(max)/λ(em)(max) 588/635 nm), have been determined at 1.8 and 1.6 Å resolution, respectively. An examination of the structures demonstrated that there are two groups of changes responsible for ...

the effects of natural zeolite as a bed medium in increasing lettuce and red tilapia growth as well as improvement of water quality parameters in an aquaponic system were investigated. an experiment with a completely randomized design was run with two treatments in triplicates, 1) an aquaponic system without use of zeolite as a control group and 2) use of a small cotton bag, containing 10 g zeo...

The dynamical system (f,R) is introduced and some of its properties are investigated. It is proven that there is an invariant set Λ on which the periodic points of f are dense.

shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of shigella have been reported. therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. attenuated live vaccines produced by gene delet...