BACKGROUND Screening of blood and blood products for human immunodeficiency virus (HIV) is routinely performed using the enzyme-linked immunosorbent assay (ELISA), and the results confirmed by Western blot (WB). However, western blot is expensive and mostly performed in developed countries. A technique more superior or comparable to WB and adaptable to developing countries must be sought. In an...

The inhibitory activity of isoferulic acid (IFA) on fructose- and glucose-mediated protein glycation and oxidation of bovine serum albumin (BSA) was investigated. Our data showed that IFA (1.25-5 mM) inhibited the formation of fluorescent advanced glycation end products (AGEs) and non-fluorescent AGE [Nε-(carboxymethyl) lysine: CML], as well as the level of fructosamine. IFA also prevented prot...

The intermediate filament protein IFA-2 is essential for the structural integrity of the Caenorhabditis elegans epidermis. It is one of the major components of the fibrous organelle, an epidermal structure comprised of apical and basal hemidesmosomes linked by cytoplasmic intermediate filaments that serve to transmit force from the muscle to the cuticle. Mutations of IFA-2 result in epidermal f...

Ifa ́ is believed to be the foundation of Yorùbá culture. This means every aspect of Yorùbá life, including religion, philosophy, science, ideology, recreation, literature and so on has one link or another with Ifa ́. Previous works on Yorùbá philosophy have neither related Ifa ́ semiotic symbols nor elements with Ayo ̀ Ọlọ́pọ ́n nor established any connection between the two. This study examines the...

Land cover changes influence the biogeochemistry, hydrology and climate of the earth. Studies that assessed land cover changes at the global scale mostly focused on: deforestation, cropland expansion, dryland degradation, urbanisation, pasture expansion and agricultural intensification. For the assessment, remotely sensed land-cover data, census data and expert knowledge were used. In tropical ...

INTENDED USE MBL Bion REAGENTS FOR IFA (Indirect Fluorescent Antibody) TESTS may be used in assays for the qualitative and/or semi-quantitative determination of specific IgG or IgM antibodies in human serum. The MBL Bion REAGENTS FOR IFA TESTS are intended for use as an aid in diagnosis of certain autoimmune diseases or for primary or active infection and/or as a determination of immunological ...

OBJECTIVE To determine sensitivity and specificity of a standardized recombinant cell-based indirect immunofluorescence assay (RC-IFA) for anti-Tr antibodies in comparison to a reference procedure. METHODS Delta/Notch-like epidermal growth factor-related receptor (DNER) was expressed in HEK293 and used as a substrate for RC-IFA. HEK293 control cells expressing CDR2/Yo and CDR2L as well as moc...