Often, we elucidate evolutionary processes backwards, starting with eutherian mammals and gradually climbing down the evolutionary tree to those species who have survived since long before mammals evolved. This is also true for elucidating the evolution of specific proteins, in this case, the protein currently known as "transthyretin" (TTR). TTR was first described in eutherian mammals and was ...

Thermostable thermolysin-like proteases (TLPs), such as the TLP of Bacillus stearothermophilus CU-21 (TLP-ste), bind calcium in one double (Ca1,2) and two single (Ca3, Ca4) calcium binding sites. The single sites are absent in thermolabile TLPs, suggesting that they are determinants of (variation in) TLP stability. Mutations in the Ca3 and Ca4 sites of TLP-ste indeed reduced thermal stability, ...

The transthyretin-like protein (TLP) from Salmonella enterica subspecies I is a periplasmic protein with high level structural similarity to a protein found in mammals and fish. In humans, the protein homologue, transthyretin, binds and carries retinol and thyroxine, and a series of other, unrelated aromatic compounds. Here we show that the amino acid sequence of the TLP from different species,...

Background Oral allergy syndrome to sapodilla plum is caused by a 21 kD allergen existing as two isoforms: an acidic thaumatin-like protein (TLP-1) and a basic thaumatin-like protein (TLP-2). The composite sequence of TLP-1 comprising the N-terminal sequence 1-7 (A-T-F-D-V/I-V-N-) and the deduced sequence 8-207 (GenBank JN624813.1) was used for this analysis. The major objective of this study w...

OBJECTIVE Carotid intima-media thickness (IMT) and plaques are markers of atherosclerosis and predict cardiovascular events. A specific sonographic triple line pattern (TLP) of the carotid wall has been identified in different conditions, but its origin and clinical significance are unclear. We examined the prevalence and predictors of TLP in a general population. METHODS AND RESULTS The stud...

PURPOSE The purpose of this study was to record transmitted-light photoplethysmography (TLP) from young permanent teeth and examine its applicability to pulp vitality assessment. METHODS Twenty-six healthy and 7 nonvital upper maxillary incisors in 17 children (ages 7 years, 3 months to 14 years, 8 months) were examined. Recordings of TLP were made with and without opaque black rubber dam app...

The turgor loss point (TLP), which is considered a threshold for many physiological processes, may be useful in plant-breeding programs or for the selection of reforestation species. Obtaining TLP through the standard pressure-volume (p-v) curve method in a large set of species is highly time-consuming and somewhat subjective. To solve this problem, we present an objective and a less time-consu...

The authors investigated whether thoracic lymphatic pumping (TLP) after FluShield vaccination enhanced the production of anti-influenza immunoglobulins in elderly individuals, who are at particular risk for influenza. Osteopathic students and non-TLP-treated elderly subjects served as controls. Serum antibody titers were quantified with enzyme-linked immunosorbent assay, and hemagglutination in...

From the first analysis of immunoprecipitation followed by Western Blotting (WB) Corin and Tumor Liberated Protein (TLP) seem to precipitate at the same height ( approximately 50KDa) and are recognized by the same antibodies. In parallel the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 are improved with the aim to be able of obtaining ...