Avian embryos and neonates acquire passive immunity by transferring maternal immunoglobulins from serum to egg yolk. Despite being a convenient source of antibodies, egg yolk immunoglobulins (IgY) from immunized hens have so far received scant attention in research. Here we report the generation and rapid isolation of IgY from the egg yolk of hens immunized against the alpha subunit of the huma...

The protective effect of hyperimmune IgY fraction of egg yolk prepared from hens hyperimmunized with multiple species of Eimeria oocysts on experimental coccidiosis was evaluated in young broilers. Chickens were continuously fed from hatch with a standard diet containing hyperimmune IgY egg yolk powder or a nonsupplemented control diet and orally challenged at d 7 posthatch with 5.0 x 10(3) spo...

The effect of non-reducing sugars (sucrose, lactose and trehalose), complex carbohydrates (cyclodextrin and dextran), infant formula or egg yolk on the stability of purified chicken IgY was evaluated under different conditions. Regardless of the protectant that was used, about 20% of the activity of IgY was lost during freeze-drying except in the presence of infant formula where the loss of IgY...

Intracellular life of Helicobacter pylori inside Candida yeast vacuole describes the establishment of H. pylori in yeast as a pre-adaptation to life in human epithelial cells. IgY-Hp conjugated with fluorescein isothiocyanate (FITC) has been previously used for identification and localization of H. pylori inside the yeast vacuole. Here we examined whether FITC-IgY-Hp internalization into yeast ...

Group A Rotaviruses are the most common cause of severe, dehydrating diarrhea in children worldwide. The aim of the present work was to evaluate protection against rotavirus (RV) diarrhea conferred by the prophylactic administration of specific IgY antibodies (Ab) to gnotobiotic piglets experimentally inoculated with virulent Wa G1P[8] human rotavirus (HRV). Chicken egg yolk IgY Ab generated fr...

The IgY-technology stand for the immunisation of chickens and the extraction of specific IgY antibodies (Ab) from egg yolk. Due to the non-invasive Ab isolation IgY-technology is internationally accepted as so-called alternative method. Despite of the aspect of animal welfare Ab production in chicken offers also outstanding economical advantages. i) purchase of chickens is much more cheep than ...

Objective It has long been known that chickens, like mammals, are capable of producing antigen-specific immunoglobulin Y (IgY), which functions similar to IgG. The present study was performed to investigate the activity of IgY anti-Mycobacterium tuberculosis on proliferation, interleukin (IL)-2, and interferon (IFN)-γ expression of rat peripheral blood mononuclear cells (PBMCs). Materials and...

Chicken egg yolk immunoglobulin (IgY) is a superior alternative to mammalian immunoglobulin. However, the practical application of IgY in research, diagnostics, and functional food is limited due to complex or time-consuming purification procedures. The objective of this study was to develop a simple, safe, large-scale separation method for IgY from egg yolk. Egg yolk was diluted with 6-fold de...

The general structure of the egg yolk antibody, containing of two light and two heavy chains, is comparable to that of mammalian immunoglobulins. Oral administration of specific egg yolk antibody is an attractive approach against gastrointestinal infection in humans and animals. The use of IgY as antibioticalternative therapy have advantages: (1) The production of IgY is a non-invasive and inex...

In Argentina Bovine Genital Campylobacteriosis is routinely diagnosed by direct immunofluorescence test. Generally, the hyperimmune sera used for this test are obtained from rabbits and less often from goats. In this work, a chicken egg yolk immunoglobulin (IgY) extract was conjugated and its ability to detect campylobacters with the regular conjugate prepared with rabbit sera was comparatively...