UMOP (Jensen 2000; Jensen & Veloso 2000; Jensen, Veloso, & Bowling 2001) is a universal planning system for multi-agent and non-deterministic domains. As input UMOP takes a planning problem described in the Nondeterministic Agent Domain Language (NADL). The NADL problem is translated to a search problem in a finite transition system. The transition system is represented by a conjunctive partiti...

Helicase/nucleoside triphosphatase (NTPase) motifs have been identified in many RNA virus genomes. Similarly, all the members of the Flaviviridae family contain conserved helicase/NTPase motifs in their homologous NS3 proteins. Although this suggests that this activity plays a critical role in the viral life cycle, the precise role of the helicase/NTPase in virus replication or whether it is es...

<p class="VARKeywords">Data sharing is a fundamental process for the advancement of both natural and social sciences. Starting from idea that computers internet have drastically changed world in last decades, this paper advocates creation space where archaeologists around can share information about maritime history exchange data with colleagues. Following principles open access, we argue...

Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copies using Light Cycler® detection system and intercalation fluorogenic dye SYBR Green I. A universal set of primers was selected from a highly con...

The expression of biotype-specific epitopes in cells infected with cytopathic (cp) and non-cytopathic (ncp) bovine viral diarrhoea virus (BVDV) was analysed by immunofluorescence. Four monoclonal antibodies (MAbs) directed against different epitopes on the viral glycoprotein gp48 were used. With cells infected with cpBVDV strain NADL, the four MAbs yielded a strong and granular cytoplasmic fluo...

Calves born persistently infected with non-cytopathic bovine viral diarrhea virus (ncpBVDV) frequently develop a fatal gastroenteric illness called mucosal disease. Both the original virus (ncpBVDV) and an antigenically identical but cytopathic virus (cpBVDV) can be isolated from animals affected by mucosal disease. Cytopathic BVDVs originate from their ncp counterparts by diverse genetic mecha...