Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6β-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna. All of t...

Atropa belladonna is a topical agent used in the treatment of skin wounds in Slovak folk medicine. The aim of this study was to asses the effect of A. belladonna extract on wound tensile strength in Sprague-Dawley rats. Twenty four rats were randomly divided into 3 groups of 8 animals. Two symmetrical skin incisions were performed on the back of each animal and immediately sutured. In the 1 gro...

Plants are a potential source of a large number of valuable secondary metabolites. In vitro cultures are being considered as an alternative to agricultural processes for studying valuable secondary metabolites. In this way, nutritive factors are important parameters influencing the production of these compounds in plants. Effects of nitrate concentrations (KNO3) on the production of two tropane...

BACKGROUND Plants are the natural source of antioxidants as well as antimicrobial compounds that has great potentials in pharmaceutical industry. In the present study, two medicinal plants Atropa belladonna and Matricaria chamomilla were collected from Northern areas of Pakistan. MATERIALS AND METHODS The extracts of the collected plants were obtained by microwave assisted extraction (MAE) wi...

The nuclear and plastid genomes of the plant cell form a coevolving unit which in interspecific combinations can lead to genetic incompatibility of compartments even between closely related taxa. This phenomenon has been observed for instance in Atropa-Nicotiana cybrids. We have sequenced the plastid chromosome of Atropa belladonna (deadly nightshade), a circular DNA molecule of 156,688 bp, and...