Abstract: The objective of this work was to evaluate the resistance transgenic papaya populations (PTPs) Papaya ringspot virus-P (PRSV-P). 'Sunrise Solo' plants were produced with gene PRSV-P protein coat, and PRSV mechanically inoculated in greenhouse conditions. presence CP/PRSV homozygosis evaluated by polymerase chain reaction (PCR). Selected control transplanted field for agronomic evaluat...

Papaya ringspot virus (PRSV), Papaya leaf distortion mosaic virus (PLDMV), and Papaya mosaic virus (PapMV) produce similar symptoms in papaya. Each threatens commercial production of papaya on Hainan Island, China. In this study, a multiplex reverse transcription PCR assay was developed to detect simultaneously these three viruses by screening combinations of mixed primer pairs and optimizing t...

Papaya is a productive and nutritious tropical fruit. Papaya Ringspot Virus (PRSV) is the most devastating pathogen threatening papaya production worldwide. Development of transgenic resistant varieties is the most effective strategy to control this disease. However, little is known about the genome-wide functional changes induced by particle bombardment transformation. We conducted transcripto...

Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter(pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-medi...

Infeksi Papaya ringspot virus (PRSV) di Indonesia pertama kali dilaporkan pada pepaya Nangroe Aceh Darussalam tahun 2012. Sejak itu, PRSV atau penyakit bercak bercincin menyebar ke beberapa daerah Jawa, Sumatera, Bali, dan Nusa Tenggara Barat. Penelitian ini dilakukan untuk mengonfirmasi keberadaan penanaman Jawa mengetahui identitas molekulernya. Metode double antibody sandwich enzyme-linked i...

UNLABELLED The protein-protein interactions between viral and host proteins play an essential role in plant virus infection and host defense. The potyviral nuclear inclusion protein a protease (NIa-Pro) is involved in various steps of viral infection. In this study, the host proteins interacting with papaya ringspot virus (PRSV) NIa-Pro were screened in a Carica papaya L. plant cDNA library usi...

Previously we showed that infection of human type II airway epithelial (A549) cells with purified respiratory syncytial virus (pRSV) induced interleukin-8 transcription by a mechanism involving cytokine-inducible cytoplasmic-nuclear translocation of the RelA transcription factor. In unstimulated cells, RelA is tethered in the cytoplasm by association with the IkappaB inhibitor and can be releas...

Controlling plant viruses by genetic engineering, including the globally important Papaya ringspot virus (PRSV), mainly involves coat protein (CP) gene mediated resistance via post-transcriptional gene silencing (PTGS). However, the breakdown of single- or double-virus resistance in CP-gene-transgenic papaya by more virulent PRSV strains has been noted in repeated field trials. Recombination an...