A problem in three-dimensional imaging by a confocal scanning laser microscope (CSLM) in the (epi))uorescence mode is the darkening of the deeper layers due to absorption and scattering of both the excitation and the uorescence light. In this paper we propose a new method to correct for these eeects. Our approach, valid for weak attenuation, consists in multiplying the measured uorescent intens...

The matching of local descriptors represents at this moment a key tool in computer vision, with a wide variety of methods designed for tasks such as image classification, object recognition and tracking, image stitching, or data mining relying on it. Local feature description techniques are usually developed so as to provide invariance to photometric variations specific to the acquisition of na...

Back propagation neural network (BPNN) algorithm is a widely used technique in training artificial neural networks. It is also a very popular optimization procedure applied to find optimal weights in a training process. However, traditional back propagation optimized with Levenberg marquardt training algorithm has some drawbacks such as getting stuck in local minima, and network stagnancy. This...

To evaluate the potential of using renewable biomass in novel HIsarna technology, reactivity thermal coal (TC), charcoal (CC), Bana grass char (BGC) before and after rapid devolatilization at 1500 °C a drop tube furnace (DTF) was investigated. Thermogravimetric (TG) used for CO2 gasification study, high temperature confocal scanning laser microscope (HT-CSLM), Brunauer-Emmett-Teller (BET) elect...

Nanoparticle complexes were prepared from chitosans of various molecular weights (MW) and degrees of deacetylation (DD). The antimicrobial effect was assessed by the Live/Dead BacLight technique in conjunction with confocal scanning laser microscopy (CSLM) and image analysis. Nanocomplexes prepared from chitosans with high MW showed a low antimicrobial effect (20 to 25% of cells damaged), where...

In this paper, a microscopic system for cell physiological research is presented. The setup which is to a large extent based on commercially available products was designed to establish a platform for time-coordinated electrophysiological and fluorescence optical compound experiments on living neurons in brain slices. Instruments for infrared differential interference contrast video microscopy ...

We attempt using simple feedforward neural networks to learn a continuous space language model (CSLM) and evaluate its performance on French and English. The training of the model is performed bilingually, in which we try to capture not only different features from one language, but also the multilingual relation demonstrated in the parallel training data. We achieved the perplexity of 191.2 on...

Photodynamic inactivation (PDI) combined with chitosan has been shown as a promising antimicrobial approach. The purpose of this study was to develop a chitosan hydrogel containing hydroxypropyl methylcellulose (HPMC), chitosan and toluidine blue O (TBO) to improve the bactericidal efficacy for topical application in clinics. The PDI efficacy of hydrogel was examined in vitro against the biofil...

Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool towards analysing diffusion processes within living biological cells. A model is presented that aims to provide a rigorous theoretical framework from which binding information of proteins from FRAP data can be extracted. A single binding reaction is considered and a set of mathematical equations is introduced...