BACKGROUND/AIMS Until recently, the cell line MDA-MB-435 was widely accepted as originating from a breast cancer. However, microarray derived data have suggested that this cell line may in fact originate from an occult melanoma. This study was designed to investigate this hypothesis further. METHODS Quantitative reverse transcription polymerase chain reaction and immunohistochemistry were use...

Several molecules that suppress metastasis without suppressing tumorigenicity have been identified, but their mechanisms of action have not yet been determined. Many block growth at the secondary site, suggesting involvement in how cells respond to signals from the extracellular milieu. Breast cancer metastasis suppressor 1 (BRMS1)-transfected MDA-MB-435 cells were examined for modifications of...

The origin of cell lines is critical in defining cell type-specific biological functions. Several reports suggested that the MDA-MB-435 cell line, a cell line extensively used for studying breast cancer biology, has a gene expression pattern most compatible with melanocyte origin. However, we demonstrate that MDA-MB-435 cells express breast-specific or epithelial-specific markers. Also, MDA-MB-...

(54) POLYAMIDES FOR NUCLEICACID CI2N IS/II (2006.01) DELIVERY A 6LX 3L/705 (2006.01) C7H 2L/04 (2006.01) (76) Inventor: Theresa M. Reineke, Blacksburg, C08G 69/48 (2006.01) VA (US) C08G 63/9. (2006.01) C7H 2L/02 (2006.01) Correspondence Address: A63L/7II (2006.01) DINSMORE & SHOHL, LLP CI2N IS/00 (2006.01) 1900 CHEMED CENTER, 255 EAST FIFTH A63L/7088 (2006.01) STREET CINCINNATI, OH 45202 (US) (...

The nanostructures and hydrophobic properties of cancer cell membranes are important for membrane fusion and cell adhesion. They are directly related to cancer cell biophysical properties, including aggressive growth and migration. Additionally, chemical component analysis of the cancer cell membrane could potentially be applied in clinical diagnosis of cancer by identification of specific biom...

OBJECTIVE Cloning of the ileal apical sodium-dependent bile acid transporter (ASBT) has identified a new pharmacological target for the modulation of plasma lipoproteins. The objective of this study was to determine whether a novel, specific, minimally absorbed ASBT inhibitor (SC-435) decreases LDL cholesterol through the alteration of plasma apoB kinetics. METHODS AND RESULTS Miniature pigs ...

HER-2/neu is a self-antigen expressed by tumors and nonmalignant epithelial tissues. The possibility of self-tolerance to HER-2/neu-derived epitopes has raised questions concerning their utility in antitumor immunotherapy. Altered HER-2/neu peptide ligands capable of eliciting enhanced immunity to tumor-associated HER-2/neu epitopes may circumvent this problem. The human CTL peptide HER-2/neu (...

Fluorescent-labeled substrates are provided for fluorescence 21 Appl. No.: 566,390 polarization assays of enzymes. These Substrates are pro 1-1. teins labeled with derivatives of BODIPY(R), 44-difluoro 22 Filed: Dec. 1, 1995 5,7-dimethyl-4-bora-3a,4a-diaza-S-indacene. The 51) Int. Cl. ........................ G01N 33/573; G01N 33/53; BODIPYOR) fluorescent tag of the present invention is pH C12O...

Discovery of the ileal apical sodium-dependent bile acid transporter (ASBT) permitted development of specific inhibitors of bile acid reabsorption, potentially a new class of cholesterol-lowering agents. In the present study, we tested the hypothesis that combining the novel ASBT inhibitor, SC-435, with the HMG-CoA reductase inhibitor, atorvastatin, would potentiate reductions in LDL cholestero...

enzymatic acidolysis of canola oil through caprylic acid was investigated to produce certain medium chain ttriacyliglycerol (tag) structured lipids (sls). lipozyme tl im, an sn-1,3 specific thermomyces lanuginosa lipase, and novozym 435, a non-specific candida antarctica lipase, were utilized as the biocatalysts in a batch reactor. reaction conditions were designed according to taguchi’s approa...