nfxC-type cells of Pseudomonas aeruginosa that produce the MexEF-OprN efflux pump exhibit resistance to fluoroquinolones and chloramphenicol and hypersusceptibility to most classical beta-lactam antibiotics. We investigated the molecular mechanism of how the nfxC mutation causes beta-lactam hypersusceptibility. The MexAB-OprM extrusion pump transports and confers resistance to beta-lactam antib...

Exposure of Pseudomonas aeruginosa to aminoglycosides frequently selects for recalcitrant subpopulations exhibiting an unstable, "adaptive" resistance to these antibiotics. In this study, we investigated the implication in the phenomenon of MexXY-OprM, an active efflux system known to export aminoglycosides in P. aeruginosa. Immunoblotting experiments demonstrated that the transporter MexY, but...

Objetivo. Describir los genes que expresan resistencia a la carbapenemasas presentes en Pseudomona aeruginosa. Métodos. Se realizó revisión de literatura científica utilizando el método PRISMA, durante febrero 2020, las bases datos Pubmed, Scopus, y Scielo. Resultados. describe reacción cadena polimerasa (PCR) como técnica más utilizada; además, se hallaron blancos moleculares hiperexpresión mú...

Simultaneous overexpression of MexAB-OprM and MexEF-OprN was demonstrated for a multiply antibiotic-resistant clinical isolate of Pseudomonas aeruginosa (G49). G49 also had decreased expression of OprF. No mutations in mexR or its upstream promoter region, mexT, oprM, oprF, or sigX were revealed, suggesting regulation by a hitherto undescribed locus.

BACKGROUND Morphine consumption can vary widely between individuals even for identical surgical procedures. As mu-opioid receptor (OPRM1) is known to modulate pain perception and mediate the analgesic effects of opioid compounds in the central nervous system, we examined the influence of two OPRM polymorphisms on acute post-operative pain and morphine usage in women undergoing elective caesarea...

Pseudomonas aeruginosa is an important opportunistic human pathogen. Certain strains can transmigrate across epithelial cells, and their invasive phenotype is correlated with capacity to cause invasive human disease and fatal septicemia in mice. Four multidrug efflux systems have been described in P. aeruginosa, however, their contribution to virulence is unclear. To clarify the role of efflux ...

Resistance mechanisms in a group of carbapenemase-negative Pseudomonas aeruginosa that were susceptible to all antibiotics except carbapenems (carbapenem resistance-only P. aeruginosa [CROPA]) were studied. Ten genetically nonrelated CROPA isolates and their carbapenem-susceptible counterparts were further investigated. OprD production was demonstrated by protein electrophoresis in only 1 of th...

One of the global health issues is antibiotic resistance in Pseudomonas aeruginosa, a causative agent bacterial infections due to multidrug (MDR), which may be mediated by efflux pumps' overexpression.The present study investigated prevalence mexXY-oprM, mexPQ-opmE genes as encoding agents pumps and determination rate clinical isolates P. aeruginosa.Different specimens infectious patients, such...

150 samples were collected from different clinical sources Al-Diwaniyah city hospitals September 2021 to January 2022. The grown on Maconkey agar and blood medium incubated at 37°C for 24 hours. Bacterial isolates diagnosed through culture characteristics, microscopic examination biochemical tests, as 25 belonging p. aeruginosa. phenotypic detection of these was conducted it found that 16 conta...