Objective: The purpose of this study was to determine if H2S is a major physiological EDHF. Methods and Results: We now show that H2S is a major EDHF because in blood vessels of CSE-deleted mice, hyperpolarization is virtually abolished. H2S acts by covalently modifying (sulfhydrating) the ATP-sensitive potassium channel, as mutating the site of sulfhydration prevents H2S-elicited hyperpolariza...

Sheng J-Z, Braun AP. Smalland intermediate-conductance Ca -activated K channels directly control agonist-evoked nitric oxide synthesis in human vascular endothelial cells. Am J Physiol Cell Physiol 293: C458–C467, 2007. First published April 25, 2007; doi:10.1152/ajpcell.00036.2007.—The contribution of small-conductance (SKCa) and intermediate-conductance Ca -activated K (IKCa) channels to the ...

Potasyum (K+) kanalları vasküler tonusun önemli düzenleyicileridir. Bu çalışmada K+ kanal tiplerinin fenilefrin ile uyarılan tonus üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Wistar Albino ırkı erkek sıçanların torasik aortlarından elde edilen 4 mm uzunluğundaki halkalar izole organ banyosu sistemine yerleştirildi. Vasküler gerim 1 grama ayarlandı. indüklenen etkilerini belirlemek için, ...

We present in this work a structural model of the open IKCa (K Ca 3.1) channel derived by homology modeling from the MthK channel structure, and used this model to compute the transmembrane potential profile along the channel pore. This analysis showed that the selectivity filter and the region extending from the channel inner cavity to the internal medium should respectively account for 81% an...

BACKGROUND AND PURPOSE Cardiovascular risk is higher in men and postmenopausal women compared with premenopausal women. This may be due to sex differences in endothelial function. Here, sex differences in endothelial function of porcine coronary arteries (PCAs) were investigated. EXPERIMENTAL APPROACH Distal PCAs were studied under myographic conditions and after precontraction with U46619. C...

Vascular stiffening, an early and common characteristic of cardiovascular diseases (CVDs), stimulates vascular smooth muscle cell (VSMC) proliferation which reciprocally accelerates the progression CVDs. However, mechanisms by extracellular matrix stiffness accompanying stiffening regulates VSMC remain largely unknown. In present study, we examined role intermediate-conductance Ca2+ -activated ...

OBJECTIVES The endothelium maintains vascular homeostasis through the release of endothelium-derived relaxing factors (EDRF) and endothelium-derived hyperpolarization (EDH). The balance in EDH : EDRF is disturbed in cardiovascular disease and may also be susceptible to developmental conditioning through exposure to an adverse uterine environment to predispose to later risk of hypertension and v...

In hair cells dissected from the frog crista ampullaris, the combination of a calcium-dependent (IKCa) and a purely voltage-dependent component (IKV) gives rise to the delayed potassium current complex (IKD). These currents have been recently reported to display slow depolarization-induced inactivation and biphasic inactivation removal by hyperpolarization. The amplitude and inactivation kineti...

BACKGROUND AND PURPOSE Increasing evidence suggests that the omega-3 polyunsaturated acids (n-3 PUFA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are beneficial to cardiovascular health, promoting relaxation of vascular smooth muscle cells and vasodilation. Numerous studies have attempted to study these responses, but to date there has not been a systematic characterisation of ...

Limiting redundancy in the real-world sensory inputs is of obvious benefit for efficient neural coding, but little is known about how this may be accomplished by biophysical neural mechanisms. One possible cellular mechanism is through adaptation to relatively constant inputs. Recent investigations in primary visual (V1) cortical neurons have demonstrated that adaptation to prolonged changes in...