In the research it was examined the changes in total phenolic contents and seven major phenolic compounds (gallic acids, +(−) catechin, catechol, Chlorogenic acid, o-coumaric acid, rutin and quercetin) of two litchi cultivars (Purbi and Bedana) shoot tips and fruits (for zygotic embryos) collected in different months, in order to determine their effects on the explants browning during establish...

A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot f...

A protocol for micrografting shoot tips harvested from in vitro shoot cultures directly to transplanted rootstock plants in the greenhouse was developed. Shoot tips of the apple (Malus domestica) cultivars Golden Delicious, Granny Smith and Fuji clone, Nagafu12, were harvested, stored in a water bath then prepared for grafting by cutting the stem immediately below the tip into a wedge shape lea...