Life has always remained a profound mystery, especially with the issue of inheritance, where no one knew how information was transmitted or stored. When it comes to stability life over huge amount time and its mutability in evolution, DNA molecule forms bigger part story than any individual might have thought. The discovery is considered passport all mysteries present challenges health, agricul...

Oligonucleotides containing cleavable linkers have emerged as versatile tools to achieve stimulus-responsive and site-specific cleavage of DNA. However, the limitations previously reported including photolabile disulfide restricted their applications in vivo. Inspired by cathepsin B-sensitive dipeptide antibody–drug conjugates (ADCs) such Adcetris, we developed Val-Ala-02 Val-Ala-Chalcone phosp...

We previously discovered one particular HLA-A*02:01 mutant that enhanced peptide-specific cytotoxic T lymphocyte (CTL) recognition in vitro compared to wild-type HLA-A*02:01. This mutant contains a single amino acid substitution from histidine to leucine at position 74 (H74L) that is located in the peptide-binding groove. To investigate the effect of the H74L mutation on the in vivo CTL priming...

Aim. To estimate the incidence of chronic kidney disease (CKD) and develop a calculator to CKD probability in patients with myocardial infarction (MI) acute injury (AKI). Material methods. A total 193 MI aged 34-79 years were examined: 123 signs AKI, 70 without AKI. In all patients, levels C-reactive protein, troponin I, N-terminal pro-brain natriuretic peptide (NT-proBNP), microalbuminuria (MA...

HLA-E shares several peptide sequences with HLA-class Ia molecules. Therefore, anti-HLA-E antibodies that recognize the shared sequences may bind to HLA-class Ia alleles. This hypothesis was validated with a murine anti-HLA-E monoclonal antibody (mAb) MEM-E/02, which reacted with microbeads coated with several HLA-B and HLA-C antigens. In this report, the hypothesis was reexamined with another ...

BACKGROUND Microparticles (MPs) are released from the plasma membrane of activated or dying cells and bear surface molecules from those cells. We examined whether donor-derived MPs in the peripheral blood of the recipient could serve as a marker of tissue damage due to rejection of a transplanted hand. METHODS Platelet-free plasma from the recipient of the transplanted hand was analyzed for M...