The metabolism of benzo(a)pyrene (BaP) by hepatic or cheek pouch epithelium microsomes obtained from Syrian golden hamsters which had been consuming an ethanol-containing liquid diet for 4 wk and from pair-fed controls was measured. Glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene or (+/-)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene as substrates was meas...

Benzo(a)pyrene (BaP) is a carcinogenic polycyclic aromatic hydrocarbon for human tissues. Still today it is not fully investigated if BaP can affect negatively the male fertility through the BaP‑DNA adducts production. In the present study, BaP Tetrol I‑1 (TI‑1) and BaP Tetrol II‑2 (TII‑2) BaP‑DNA adducts were investigated in spermatozoa of a Sicilian male population. Semen samples from 86 volu...

Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon produced by cigarette combustion, is implicated as a causative agent in smoking-related cancer and atherosclerosis. 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3], a potent ligand for the nuclear receptor vitamin D receptor (VDR), has been shown to decrease the risk of osteoporosis, some types of cancer and cardiovascular disease, suggesting an op...

Benzo(a)pyrene (BaP), a primary component of tobacco smoke, is activated by cytochrome P450 1B1 (CYP1B1). Smokers homozygous for the C-allele (*1/*1) at the CYP1B1 Leu432Val polymorphism have shown increased CYP1B1 expression, compared to smokers homozygous for the G-allele *3/*3. Since no difference has been shown in CYP1B1 expression between both genotypes in non-smokers, we assumed that the ...

Benzo[a]pyrene (BaP) activates the aryl hydrocarbon (AHR) and induces the expression of genes involved in xenobiotic metabolism, including CYP1A1. CYP1A1 is involved not only in BaP detoxification but also in metabolic activation, which results in DNA adduct formation. Vitamin D receptor (VDR) belongs to the NR1I subfamily of the nuclear receptor superfamily, which also regulates expression of ...

In vitro organogenesis of citrus was studied for the genotypes Citrus sinensis cv. ‘Natal’, C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP – 0.0; 0.5; 1.0; 1.5, or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. auranti...

This experiment was conducted to investigate the effects of Bacillus amyloliquefaciens probiotic (BAP) as a direct-fed microbial on growth performance, cecal microflora, serum immunoglobulin levels, and fecal noxious gas emissions of broiler chickens. A total of 400 one-day-old broiler chicks (Ross 308) were randomly assigned to 1 of 5 treatment diets formulated to supply 0, 1, 5, 10, and 20 g/...

It is believed that the formation of DNA adducts is an essential initial step in carcinogenesis. To better understand this initial step and its relationship to cancer, this study primarily focuses on benzo(a)pyrene (BaP), a highly carcinogenic model polycyclic aromatic hydrocarbon (PAH) and an ubiquitous environmental contaminant. The objective of this dissertation was to identify and quantify ...

The incidence of bronchogenic carcinoma is increased substantially in asbestos workers who smoke. We used several approaches to determine possible mechanisms of synergism at the cellular level between asbestos and the polycyclic aromatic hydrocarbon (PAH), benzo(a)pyrene (BaP), a chemical carcinogen in cigarette smoke. Specifically, we hypothesized that cellular uptake and metabolism of BaP mig...

Abstract: Cacari is a fruit tree that native to the Amazon region of Brazil. The considered have highest vitamin C content any edible (13.757 mg 100 g-1 dry weight). objective this study was evaluate effects 2,4-D and 6-BAP alone or in combination on vitro callus induction pro-embryogenic mass formation cacari stem segments different culture media as precursor for micropropagation. experiment c...