نتایج جستجو برای: 1 bap and 02 mg l
تعداد نتایج: 17355727 فیلتر نتایج به سال:
تکنیک کشت بافت یا ریزازدیادی جهت تکثیر انبوه بسیاری از گیاهان به کار می رود. با استفاده از این تکنیک می توان گیاهان سالم و عاری از ویروس را تولید نمود. گل کاغذی یکی از گیاهان زینتی سازگار به آب و هوای گرم و خشک می باشد اما تکثیر آن به علت محدودیت قلمه های مناسب، نیاز به هورمون ها و سیستم مه پاش جهت تکثیر ارقام سخت ریشه زا، پایین بودن درصد موفقیت تکثیر با قلمه و عدم تولید بذر با مشکل مواجه است. ...
Due to the problems of sexual and asexual reproduction of Haworthia attenuatae as one of the most popular ornamental succulents, the possibility of micropropagation of this plant in in vitro conditions was studied. The effect of BAP (Benzylamino purine) and Kin (Kinetin) (0.5, 1 and 2 mg/l) in Combination with IBA (Indole-3-acetic acid) (0, 0.01, 0.1 and 0.5 mg/l) using leaf explants on the sho...
A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared e...
گیاه دارویی عناب دارای ارزش اقتصادی زیادی است اما روشهای سنتی تکثیر این گیاه زمان زیادی نیاز دارد و گیاهان تکثیر شده از نظر سلامت تضمین شده نمی باشند درحالی که روشهای تکثیر در شرایط درون شیشه ایی در زمان کوتاه امکان تولید انبوه تعدادزیادی از گیاهان عاری ازبیماری را فراهم می آورند. هدف از تحقیق حاضر بهینه سازی پروتکل ریزازدیادی دو اکوتیپ بومی(قاهان قم و گیوک بیرجند) گیاه عناب بود. ریزنمونه ها ...
In vitro propagation of three Iranian apricot cultivars, “Ghavami”, “RajabAli” and “Khiveei” was studied by direct micropropagation technique. To optimize sterile manipulation, shoot nods of one year-old dormant vegetative shoots and current growing vegetative shoot were cultured in WPM medium after treatment by HgCl2, citric acid, ethanol and NaClO (10, 15, 20 min). Establishment of buds was t...
the effect of in planta tiba and proline on in vitro seedlings and cell culture of sugar beet was investigated. sterilized seeds were grown in vitro on ½ strength ms medium supplemented with 0 or 3 mg/l tiba. calli obtained on young leaves cultured on ms medium containing 1 mg/l bap, were used for the intiation of cell suspension cultures using ms basal composition supplemented with 0 or 50 m m...
In vitro propagation of C. morifolium cv. ‘Homa’ and cv. ‘Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0.05, 0.1 and 0.2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the callu...
An efficient embryogenic response (both direct and indirect) of two different varieties of sugarcane (CP 77,400 and BL-4) was obtained in this study. The results demonstrates that fresh inner whorl of leaves and shoot apical meristem of sugarcane were highly amenable to in vitro somatic embryogenesis (both direct and indirect). A combination of 3.0 mg/l 2,4-D with 0.25 mg/l BAP was more potent ...
Purslane (Portulaca oleracea L.) contains valuable secondary metabolites such as Dopamin, Noradrenaline and Omega-3. This plant is used in various medicinal, food and hygienic industries as well as the treatment of different diseases such as diabetes, heart disease and pain relief. Callus induced from medicinal plants are used to increase the production of secondary metabolities in cell suspens...
An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed ...
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