We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interact...

80 S ribosomes and elongation factors 1 and 2 (EF-1 and EF-2), isolated from freeze-dried porcine brain of the hypothalamic region, were used to study the interrelation between the ribosomal binding of the two factors and of phenylalanyltRNA and N-acetyl-phenylalanyl-tRNA. Complexing of ribosomes with EF-2 + /3, y-methylene-guanosine S’-triphosphate or EF-2 + GTP + fusidic acid prevented or inh...

let g be a (p, q) graph. let k be an integer with 2 ≤ k ≤ p and f from v (g) to the set {1, 2, . . . , k} be a map. for each edge uv, assign the label |f(u) − f(v)|. the function f is called a k-difference cordial labeling of g if |νf (i) − vf (j)| ≤ 1 and |ef (0) − ef (1)| ≤ 1 where vf (x) denotes the number of vertices labelled with x (x ∈ {1, 2 . . . , k}), ef (1) and ef (0) respectively den...

To evaluate the clinical usefulness of the first-third ejection fraction (1/3 EF) for detecting patients with coronary artery disease (CAD), resting contrast ventriculography and first-pass radionuclide angiography with a high-count-rate, multicrystal camera system were performed in 47 subjects: 22 normal controls (group 1) and 25 patients with clinically stable angina pectoris and severe CAD (...

A procedure for the purification of elongation factor 1 (EF-1) from KrebsII mouse ascites cells is described. Ascites EF-1 occurs in multiple forms of different molecular weight like the corresponding enzymes from calf brain and rat liver. Biogel A-5m chromatography of our purest EF-1 resulted in a pattern indicating the presence of different molecular weight components. However, only a single ...

background : airborne particulate matter is one of the most important atmospheric pollutants. tile sources of particulate matter in the atmosphere are divided into two groups; 1) natural sources and 2) anthropogenic sources. the important components of airborne particulate matter and invarying concentrations are the ubiquitous components of the lower atmosphere. heavy metals are mainly emitted ...

Genes encoding two proteins corresponding to elongation factor G (EF-G) were cloned from Pseudomonas aeruginosa. The proteins encoded by these genes are both members of the EFG I subfamily. The gene encoding one of the forms of EF-G is located in the str operon and the resulting protein is referred to as EF-G1A while the gene encoding the other form of EF-G is located in another part of the gen...

In order to assess the limitations imposed on a cochlear implant system by a wearable speech processor, the parameters extracted from a set of 11 vowels and 24 consonants were examined. An estimate of the fundamental frequency EF 0 was derived from the zero crossings of the low-pass filtered envelope of the waveform. Estimates of the first and second formant frequencies EF 1 and EF 2 were deriv...