the b-cell cd20 antigen is one of the most reliable surface targets in immunotherapy of b lymphoma. in this project, we studied the production and characterization of a new monoclonal antibody against chimeric human cd20 extra loops (hcd20 exl). the results showed that clone c12h, igg2/k isotype reacted with the antigen in elisa and immunoblot. the kd value was  found to be 2×10-9m and flow cyt...

Despite the various therapies available, the use of monoclonal antibodies is a highly specific approach that has only recently been of interest to researchers. The properties of antibodies have led to their use in the treatment of various diseases, including cancer, Alzheimer's disease, diabetes and multiple sclerosis (MS). MS, a chronic inflammatory disease, occurs commonly in young adults. Th...

background: monoclonal antibodies have been employed extensively for the identification of leishmania species, development of diagnostic tests and in the characterization of defined leishmanial antigens. objectives: identification and characterization of  leishmania spp. directly from cutaneous lesions of infected individuals. methods: an immunoperoxidase test (avidin-biotin technique) using mo...

alpha-fetoprotein (afp), a serum glycoprotein belonging to the onco-developmental proteins group, serves as a marker both in cancer research and in studies concerning fetal development and fetal pathophysiology. monoclonal anti-afp antibodies are essential reagents in developing appropriate techniques for measurement of this protein. in this study, in order to produce anti-afp monoclonal antibo...

both zoonotic and anthroponotic cutaneous leishmaniasis (cl) caused by l. major and l. tropica, respectively, are endemic in different parts of iran. this study was performed to investigate the new changes in epidemiological pattern of cl, and to identify the species of leishmania and the strains of l. major isolated from different endemic areas of iran. seventy-two isolates from 245 samples co...

background: different methods have been used for characterization of leishmania promastigotes. monoclonal antibodies are useful in characterization of   leishmania spp . both amastogotes and promastigotes. objective: comparing the characterization of   leishmania spp. promastigotes with immunoperoxidase test (avidin-biotin) techniques and an indirect immunofluorescent assay (ifa).   methods: ap...

A monoclonal antibody has been developed against the type II PtdIns 4-kinase from bovine brain. This antibody, 4C5G, causes greater than 90% inhibition of the type II PtdIns 4-kinase from bovine brain, rat brain and human erythrocytes. However, it fails to inhibit type III PtdIns 4-kinase from bovine brain or PtdIns 3-kinase from rat liver. These results suggest that type II and type III PtdIns...

The presence of a low level of M protein in the peripheral blood <3gm/dl which is below the required cut off for the diagnosis of plasma cell myeloma is known as monoclonal gammopathy of undetermined significance (MGUS). Elevated levels of serum M protein and detection of M band on serum protein electrophoresis (SPE) can occur in chronic diseases not related to B cell disorders and not always d...