one hundred and fifty blood samples were prepared from cattle of a region in isfahan. the extracted dna from blood cells were analyzed by a. centrale (amori strain) specific nested-pcr using primers derived from 16s rrna gene. all blood smears were negative for a. centrale like structures. the results showed that 2 of total 150 blood samples (1.33%) were a. centrale (amori strain) positive by s...

A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia...

In this study, to increase information about the relationship between caprine and ovine isolates of Pasteurella multocida, 16S rRNA gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. Also, capsular type and toxAgene presentation was studied in this paper. All isolates, except one, belong to capsular type A, and toxA+ strain distributed among strains were isolated from both...

uricase or urate oxidase (urate:oxygen oxidoreductase, ec 1.7.3.3), a peroxisomal enzyme which is found in many bacteria, catalyzes the oxidative opening of the purine ring of urate to yield allantoin, carbon dioxide, and hydrogen peroxide. in this study, the phylogeny of urate oxidase (uricase) producing bacteria was studied based on gene sequences of 16s rrna and uricase protein. representati...

Several characteristics of the 16S rRNA gene, such as its essential function, ubiquity, and evolutionary properties, have allowed it to become the most commonly used molecular marker in microbial ecology. However, one fact that has been overlooked is that multiple copies of this gene are often present in a given bacterium. These intragenomic copies can differ in sequence, leading to identificat...

Abstract Faeces from cows with different milk yield and non-lactating were applied to soil investigate whether N 2 O efflux is related feeding-induced differences in faecal microbiome abundances of nitrification denitrification genes. Fungal 18S-rRNA gene abundance was the highest faeces group. The 18S-rRNA/ergosterol ratio showed a strong positive correlation 18S-rRNA/fungal glucosamine ratio....

16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryoti...

BACKGROUND Broad-range 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) is used for detection and identification of bacterial pathogens in clinical specimens from patients with a high suspicion for infection. However, prospective studies addressing the impact and clinical value of broad-range bacterial 16S rRNA gene amplification for diagnosis of acute infectious diseases in nonsel...

API 20E strip test, the standard for Enterobacteriaceae identification, is not sufficient to discriminate some Yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g. Yersinia ferderiksenii and Yersinia intermedia, which need a variety of molecular biology methods as auxiliaries for identification. The 16S rRNA gene is considered...