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We report the nonenzymatic terminal labeling of oligoribonucleotide (ORN) by using template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine ((CV)U) with high selectivity.
The development of economical de novo gene synthesis methods using microchip-synthesized oligonucleotides has been limited by their high error rates. In this study, a low-cost, effective and improved-throughput (up to 32 oligos per run) error-removal method using an immobilized cellulose column containing the mismatch binding protein MutS was produced to generate high-quality DNA from oligos, p...
introduction: β-carbolines are shown to have significant anti-inflammatory effect via the inhibition of some inflammatory mediators including tnf-α and pge2. in previous studies purkinje cell deterioration have been proposed the dominant pathogenesis of harmaline toxicity. win55, 212-2 is a non-selective cannabinoid cb1 and cb2 receptor agonist. combination of win55, 212-2 and am 251(cb1-select...
[formula: see text] 5-Amino-2'-deoxyuridine 5'-triphosphate, an analogue of deoxythymidine triphosphate, was synthesized and found to be a substrate of Taq DNA polymerase. The DNA-borne analogue underwent selective chemical reaction with permanganate. The use of 5-amino-dU as an interference probe was validated using the Ada protein/ada promoter complex. The performance of 5-amino-dU in interfe...
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