نتایج جستجو برای: 5utr region nested pcr rt
تعداد نتایج: 742220 فیلتر نتایج به سال:
Background and Aims: lamivudine is amongst the antiviral for drug chronic hepatitis B treatment. During therapy with lamivudine, variants may emerge with YMDD mutation in the reverse transcriptase (RT) region of polymerase gene. This mutation might have a role in drug resistant for HBV. Materials and Methods: HBV DNA extraction from serum sample of 88 patients, were subjected to nested PCR for ...
BACKGROUND AFRIMS longitudinal dengue surveillance in Thailand depends on the nested RT-PCR and the dengue IgM/IgG ELISA. OBJECTIVE To examine and improve the sensitivity of the nested RT-PCR using a panel of archived samples collected during dengue surveillance. STUDY DESIGN A retrospective analysis of 16,454 dengue IgM/IgG ELISA positive cases collected between 2000 and 2013 was done to i...
During the period from July 2002 to June 2003, infectious bursal disease (IBD) was suspected in 101 commercial flocks of broiler chickens on the basis of clinical and post mortem findings in some districts of the Haryana state, India. Bursal samples were collected randomly from 20 flocks for the detection of infectious bursal disease virus (IBDV) by reverse transcriptionpolymerase chain reactio...
در این مطالعه یک روش Nested-PCR برای تشخیص دو ویروس BVDV از سویه NADL بهینه سازی گردید. در آزمایش RT-PCR، قسمتی از منطقه غیر قابل ترجمه ناحیه '5 ویروس به طول 249 جفت باز تکثیر شد. محصول PCR در وکتور R/T57pTZ کلون گردید و نتیجه تعیین ردیف اسیدهای نوکلوتیدی آن اختصاصی بودن آزمایش را تایید نمود. سپس پرایمرهای داخلی انتخاب و آزمایش Nested-PCR انجام گردید و یک قطعه DNA به طول 155 جفت باز تکث...
For detecting Borna disease virus (BDV) genomic stranded RNA, single-tube reverse transcription-polymerase chain reaction (St RT-PCR) was developed to equal the sensitivity of RT-nested PCR but with reduced risk of contamination. BDV-genomic stranded RNA was synthesized in vitro using plasmid cDNA of BDV p24 region as a template and RNA was also extracted from BDV-persistently infected MDCK (MD...
In this article, the development of a new TaqMan-based one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection and quantification of Crimean-Congo hemorrhagic fever virus (CCHFV) RNA is described. Selected oligos targeting the highly conserved S region of CCHFV were designed by using our oligo design and analysis software, Oligoware 1.0. None of the prime...
Viral infections cause important problems and significant economic loses in sheep and goats that can be protected by an investigation of infections. This study aimed to determine the sheep and goat viral infections including bluetongue virus (BTV), maedivisna virus (MVV) and caprine arthritis-encephalitis virus (CAEV) in Kirikkale city located in Central Anatolia region of Turkey. Blood samples...
A traditional nested reverse transcription-polymerase chain reaction (RT-PCR) assay specific for eastern equine encephalomyelitis (EEE) virus was designed to multiplex with a previously described West Nile (WN) virus nested RT-PCR assay. Differentiation of EEE and WN was based on base pair size of the amplified product. One hundred fifty-seven mammalian and avian brain tissues were tested by EE...
This study evaluated and compared the performance of two real-time PCR assays using nested reverse transcription (RT)-PCR as the reference method. Among 117 nested RT-PCR-positive cases, the abTES DEN 5 qPCR kit detected 97.4% of dengue virus (DENV) infections, while the innuDETECT Dengue TwoStep assay did so for 44.4%. Sensitivity varied by infecting serotype and the stage of infection. The ab...
در این مطالعه یک روش nested-pcr برای تشخیص دو ویروس bvdv از سویه nadl بهینه سازی گردید. در آزمایش rt-pcr، قسمتی از منطقه غیر قابل ترجمه ناحیه '5 ویروس به طول 249 جفت باز تکثیر شد. محصول pcr در وکتور r/t57ptz کلون گردید و نتیجه تعیین ردیف اسیدهای نوکلوتیدی آن اختصاصی بودن آزمایش را تایید نمود. سپس پرایمرهای داخلی انتخاب و آزمایش nested-pcr انجام گردید و یک قطعه dna به طول 155 جفت باز تکثیر شد. م...
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