Background: The monoclonal antibody, C595, against breast cancer cell line was conjugated with cyclic anhydride gadolinium-diethylenetriaminepenta-acetic acid (Gd-cDTPAa) to produce Gd-DTPA-C595 and used as specific breast cancer cell line (MCF-7) contrast agents in magnetic resonance imaging (MRI).  Methods: After incubation of breast cancer cell line (MCF-7), with different contrast agents (G...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

objective: the study of physiological changes in recombinant cell lines provides useful information to improve production performance. in this study, we investigate the effects of an anti-cd33 chimeric igg4 expression on sp2.0 cell growth. methods: variable region genes of light and heavy chains of monoclonal antibody produced by m195 were cloned in pfuse-clig-hk and pfuse-chig-hg4 expression ...

background: both azadirachtin and rotenone, two botanical pesticides have shown inhibit the growth of cultured insect cells. the inhibitory effect of rotenone on cell growth is through disruption of electron transport chain, but exact mechanism of effect of azadirachtin on growth of cell is unknown. this study was carried out to compare the effect of these phytochemical on oxygen consumption by...

background: most cancer cells become resistant to anti-cancer agents. in the last few years, a new approach for targeted therapy of human cancer has been developed using immunotoxins which comprise both the cell targeting and the cell killing moieties. methods: in the present study, the recombinant shiga toxin a1 subunit fused to human granulocyte-macrophage colony stimulating factor (a1-gm-csf...

in this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in iran. the cells were originating from two-day-old normal male calf of holstein breed. the cell cultures were continuously passaged following complete proliferation of primary cells. the specific properties or characteristics of the cell were defined using cytogene...

the aim of this study was to develop a novel multifunctional nanoparticle, which encapsulates spion and gemcitabine in plga±peg to form multifunctional drug delivery system. for this aim, super paramagnetic iron oxide nanoparticles (spions) were synthesized and encapsulated simultaneously with gemcitabine (gem) in plga±peg copolymers via w/o/w double emulsification method. optimum size and enca...

background: the rh antigens are expressed as parts of a protein complex in the rbc membrane. this complex is a tetramer, consisting of two molecules of rhag and two molecules of rh proteins. to express rhd in rbc membrane, expression of rhag is essential. this co-expression only occurs in the erythroid lineage. k562 cell line has an erythroid lineage. materials and methods: cord blood was used ...