Despite the power of model systems to reveal basic immunologic mechanisms, critical differences exist between species that necessitate the direct study of human cells. Illustrating this point is the difference in phenotype between patients with SCID caused by mutations affecting the common γ-chain (γc) cytokine signaling pathway and mice with similar mutations. Although in both species, null mu...

Soluble interleukin-7 (IL-7) receptor α (sCD127) is implicated in the pathogenesis of autoimmune diseases. We show that serum sCD127 concentrations are increased at the onset of type 1 diabetes (T1D; n = 390) as compared with concentrations in age-matched islet autoantibody-negative first-degree relatives of patients (n = 392; P = 0.00001). sCD127 concentration in patients was influenced by isl...

Interleukin-7 (IL-7) concentrations are increased in the blood of CD4+ T cell depleted individuals, including HIV-1 infected patients. High IL-7 levels might stimulate T cell activation and, as we have shown earlier, IL-7 can prime resting T cell to CD95 induced apoptosis as well. HIV-1 infection leads to B cell abnormalities including increased apoptosis via the CD95 (Fas) death receptor pathw...

Let a,ß > -1 and Pla'3)(x) = (l-x)a(l+x)ßp[a'a){x), where Pjf (x) is the Jacobi function of the first kind, A > —(a + ß + l)/2, and -1 < i < 1. Let r the integral exists. It is known that for a + •>-Ä4r'<",')(*-i),£Ä<-> *J o whenever ß = 0, we have /(*) = lim 4 f fC« (x l) PÍÍ$A-*)\ . . r2(A + i/2) X sin 7rA^-:-L_¡-d\ w in J, r(A-ra + l/2)F(A + ^ + l/2) almost everywhere in [—1,1], In this pape...

BACKGROUND AND OBJECTIVES Interleukin-7 (IL-7) has been studied for its possible immunorestorative capacities following stem cell transplantation and has been shown to enhance post-transplant immune recovery predominantly by peripheral T-cell expansion. A major concern of IL-7 is its possible aggravating effect on graft-versus-host and host-versus-graft reactivity. DESIGN AND METHODS To study...

Abstract Common lymphoid progenitors (CLPs) in the bone marrow give rise to both T and B cells, but mechanisms underpinning downstream cell fate decisions have yet be fully elucidated. We identified N-Myc Downstream Regulated Gene 3 (NDRG3) - a highly conserved regulator of Wnt/B-catenin signaling as novel lymphocyte substrate Akt kinase. To determine effect NDRG3 phosphorylation, hematopoietic...

Plasmacytoid dendritic cells (pDCs) competent to make type I interferon were rigorously defined as a Ly-6C(+) and CD11c(Lo) subset of the B220(+)CD19(-) CD43(+)CD24(Lo) bone marrow (BM) Fraction A. Otherwise similar Ly6C(-) cells expressed the natural killer (NK) markers DX5 and NK1.1. pDCs represented a stable, discrete, and long-lived population. Stem cells and early lymphoid progenitors (ELP...

Transgenic and gene targeted mice have contributed greatly to our understanding of the mechanisms underlying B-cell development. We describe here a model system that allows us to apply molecular genetic techniques to the analysis of human B-cell development. We constructed a retroviral vector with a multiple cloning site connected to a gene encoding green fluorescent protein by an internal ribo...

Multiple sclerosis (MS) is a chronic inflammatory disease with a substantial genetic component. Since the discovery of anti-aquaporin-4 (AQP4) antibodies, a specific biomarker for neuromyelitis optica (NMO), NMO has been classified as a distinct disease entity with a fundamentally different etiology from MS. Recent genetic studies have clarified the distinct and common genetic profiles of Japan...