In the present paper, we report on enzyme-initiated self-assembly of Fmoc (fluoren-9-ylmethoxycarbonyl)-tyrosine hydrogels by enzymatic dephosphorylation under physiological conditions and provide evidence for the ability to control the modulus. Upon enzyme action, a self-assembling network of interconnecting fibres is formed, observed by cryo-SEM (scanning electron microscopy) and TEM (transmi...

Abstract The accumulation of organophosphorus type herbicides has been observed worldwide in the environment (i.e. soil, water), together with their appearance foods plant origin. This paper reports a new liquid chromatography–isotope dilution–tandem mass spectrometric method (LC-IDMS) for analysis glufosinate (GLUF), glyphosate (GLY) and its main metabolite, aminomethylphosphonic acid (AMPA), ...

Synthetic materials designed for improved biomimicry of the extracellular matrix must contain fibrous, bioactive, and mechanical cues. Self-assembly low molecular weight gelator (LMWG) peptides Fmoc-DIKVAV (Fmoc-aspartic acid-isoleucine-lysine-valine-alanine-valine) Fmoc-FRGDF (Fmoc-phenylalanine-arginine-glycine-aspartic acid-phenylalanine) creates fibrous bioactive hydrogels. Polysaccharides ...

The covalent linkage of peptides or protein drugs to human serum albumin (HSA) greatly prolongs their lifetime in vivo but is pharmacologically irrelevant when it irreversibly inactivates them. We retain drug bioactivity by synthesizing a heterobifunctional reagent (MAL-Fmoc-OSu, 9-hydroxymethyl-2-(amino-3-maleimidopropionate)-fluorene-N-hydroxysuccinimide) that generates HSA-Fmoc-insulin on co...

A novel synthetic N-(9-fluorenyl methoxy carbonyl)-L-Cysteine (Fmoc-Cys(SH)-OH) receptor was prepared by co-polymerizing (9-fluorenyl methoxy carbonyl)-S-(1-propene-2-thiol)-L-Cysteine (FmocCys(SCH2CHCH2)-OH) and a non-imprinted polymer prepared from 1-propene-1-thiol photo-chemically 15 h at room temperature and additional 3 h thermally at 80 ̊C. Subsequently, disulfides were reduced with lithi...

Abbreviations used: Acm, acetamidomethyl; Boc, tert-butoxycarbonyl; Bzl, benzyl; Bzl(4-Me), 4-methylbenzyl; DPDS, di(2-pyridyl)disulfide; DMSO, dimethylsulfoxide; Fmoc, 9-fluorenylmethoxycarbonyl; Mob, 4-methoxybenzyl; Npys, 3-nitro-2-pyridylsulfanyl; Pys, 2-pyridylsulfanyl; StBu, tert-butylsulfanyl; sec, selenocysteine; tBu, tert-butyl; TFA, trifluoroacetic acid; TfOH, trifluoromethanesulfonic...

FMOC-Cl is a derivatizing agent widely used both in N-protection reactions of a-amino acids as well quantification and detection amino related molecules using HPLC-PDA or fluorescence analysis. In this work we studied the reaction with gabapentin. However, it was observed that degradation gabapentin-N-FMOC to FMOC-OH occur simultaneously presence basic medium which specific for gabapentin deriv...

2-Methoxy-4,4’-bis(methylthio)benzhydrylamine (10) and the corresponding disulfoxide were prepared and tested as a model amide protecting groups for their stability toward acidic conditions. Subsequently, the novel 4-[4,4’-bis(methylsulfinyl)-2-o~-(9-fluorenylmethylo~c~bonyl) benzhydrylaminolbutanoic acid (SCAL) handle (9) has been prepared and applied to solid-phase peptide synthesis of C-term...

The noncoded aromatic 3,4-dihydroxy-L-phenylalanine (DOPA) amino acid has a pivotal role in the remarkable adhesive properties displayed by marine mussels. These properties have inspired the design of adhesive chemical entities through various synthetic approaches. DOPA-containing bioinspired polymers have a broad functional appeal beyond adhesion due to the diverse chemical interactions presen...

The enantiomeric determination of amphetamine and various amphetamine-type compounds by liquid chromatography after chiral derivatization with 9-fluorenylmethyl chloroformate-L-proline (FMOC-L-Pro) is reported. The results obtained were compared with those achieved after achiral derivatization with 9-fluorenylmethyl chloroformate and subsequent separation of the derivatives on a beta-cyclodextr...