The effects of hexosamines and their acetyl derivatives on the aggregation of 4-dimethylaminoazobenzene-induced rat hepatoma cells, dRLa-74 and dRLh-84, which have a low and high tumor-producing activity, respectively, were examined in rotation-mediated cell culture. D-Glucosamine, D-galactosamine, and D-mannosamine had relatively little effect on aggregation of either strain of hepatoma cells ...

INTRODUCTION Little is known about the early events in the interaction between Paracoccidioides brasiliensis and its host. To understand the effect of carbohydrates in the interaction between the fungus and epithelial cell in culture, we analyzed the influence of different carbohydrate solutions on the adhesion of P. brasiliensis yeast cells to CCL-6 cells in culture. METHODS Fungal cells wer...

Azoproteins prepared with p-aminophenyl-beta-N-acetyl-glucosaminide react in precipitin tests with Group A streptococcal antisera. The reaction is nonreciprocal, and antisera to the azoprotein do not react with Group A carbohydrate. Phenyl-N-acetyl-glucosaminides inhibit the reaction of Group A carbohydrate with homologous antisera and with antisera to the azoprotein. The beta-anomer is more ef...

To successfully colonize and persist within a host niche, bacteria must properly regulate their gene expression profiles. The marine bacterium Vibrio fischeri establishes a mutualistic symbiosis within the light organ of the Hawaiian squid, Euprymna scolopes. Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-acetyl-D-glucosamine-utilization genes that...

The genetic relationships between the multiple variants of mucolipidosis II (I-cell disease) and mucolipidosis III (pseudo-Hurler polydystrophy) were investigated with a sensitive genetic complementation analysis procedure. These clinically distinct disorders have defects in the synthesis of a recognition marker necessary for the intracellular transport of acid hydrolases into lysosomes. Both d...

Glycans of cell surface glycoproteins are involved in the regulation of cell migration, growth, and differentiation. N-acetyl-glucosaminyltransferase V (GnT-V) transfers N-acetyl-d-glucosamine to form β1,6-branched N-glycans, thus playing a crucial role in the biosynthesis of glycoproteins. This study reveals the distinct expression of GnT-V in STRO-1 and CD-146 double-positive dental pulp stem...

fermentation of 49 carbohydrates and oxidation and assimilation of 96 substrates were examined for ten beauveria bassiana isolates. principal component analysis indicated that there are significant differences among isolates for utilization of some substrates. esculine and d- glucose were positively fermented by all isolates. isolates were grouped into four clusters on the basis of hierarchial ...

Lipopolysaccharides (LPS) from two enteropathogenic strains of E. coli O142 and O158 were isolated by hot phenol-water extraction procedure. Polyacrylamide gel electrophoretic pattern of the LPS showed the typical ladder like pattern of smooth type of LPS. The LPS of E. coli O158 was found to contain L-rhamnose, D-glucose and N-acetyl-D-galactosamine as major constituents together with D-galact...

Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.

the amphiphilic graft copolymer using chitosan (cs) as hydrophilic segment and poly (l-lactic acid) (plla) as hydrophobic segment, was prepared through a protection-graft-de protection route. chitosan is a polysaccharide comprising of copolymers of glucosamine and n-acetyl glucosamine. chitosan is the deacetylated derivative of chitin, which is one of the most abundant natural polysaccharides c...