Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

In view of the importance of a rapid aetiological diagnosis in septicaemia, we compared the results of subculture, Gram staining and acridine orange staining in the detection of positive blood cultures. The study was based on 1013 blood cultures of which 138 were positive by culture. The three techniques were applied 12 h after the specimen was taken in 210 instances, at 24 h in 540 instances a...

The recent paper by Rotimi and colleagues 1 does not mention the acridine orange stain 2 when comparing staining methods for the identification of Helicobacter pylori. The acri-dine orange stain uses ultraviolet fluores-cence in the identification of bacteria. The typical curved morphology of H pylori can easily be diVerentiated from other bacteria. I have used this quick, cheap, and reliable s...

introduction: date palm gemmule has been used in traditional medicine to improve the semen quality and treat infertility. their components remedies those are rich of antioxidant and vitamins can influence spermatogenesis. the aim of this project was to evaluate the effects of date palm gommule water/alcoholic extract on reproductive tissues, sex hormone and sperm quality of rat. methods: sevent...

p-nonylphenol (p-np) as a non ionic surfactant is used in many industries such as pesticides, cosmetics,detergents and so on. our previous study showed that the p-np causes reduction in viability of the rats bonemarrow mesenchymal stem cells (mscs). the aim of the present study was to investigate the mechanism of the cell death due to p-np exposure. the cells, after 3rd passage were treated wit...