In the presence of actinomycin D or a combination of actinomycin D and either camptothecin or alpha-amanatin. Aedes albopictus cells synthesize a variety of single stranded RNA species. These actinomycin D resistant species are ethidium bromide sensitive and they are present in the cell cytoplasm in an RNase resistant structure which has the sedimentation and buoyant density characteristics of ...

The effects of actinomycin D on RNA and protein synthesis have been investigated in Tetrahvmena twiformis. The extent of inhibition of total RNA svnthesis and the time before inhibition occurs & both dependent on the concentration of the drug over the range OS-10 pg/ml. In contrast to reports on mammalian systems, actinomycin D at low doses is not a selective inhibitor of rRNA synthesis in Tetr...

The technique of photoaffinity labeling is applied to the actinomycin D system to provide a novel probe for the examination of the interactions of actinomycin D with nucleic acids. The capacity for covalent attachment of actinomycin D will aid greatly in the study of target-site specificities and the correlations of biological effects with biophysical DNA interactions. Through chemical modifica...

A single mutation in Saccharomyces cerevisiae conferred sensitivity to low concentrations of actinomycin D. Treatment with actinomycin D preferentially inhibited synthesis of rRNA's. Residual rRNA synthesized was processed normally. Total protein synthesis and inducibility of the enzyme maltase were relatively unaffected at concentrations of actinomycin D which severely inhibited rRNA synthesis.

“Superinduction” is a phenomenon frequently observed in eukaryotic cells, whereby the inhibition of RNA synthesis (usually with actinomycin D) results in an increase in the concentration (activity) of a specific protein. This paper shows that actinomycin D can superinduce the major egg white proteins synthesized in chick magnum. After actinomycin D treatment, ovalbumin, conalbumin, ovomucoid, a...

background: melatonin, a reactive oxygen species (ros) scavenger and an antioxidant, has been shown that can inhibit apoptosis. administration of melatonin may improve embryo development in assisted reproductive technology (art). objective: the aim of this study was to evaluate the role of melatonin in inhibition of spontaneous and induced apoptosis by tumor necrosis factor alph (tnf-α) and act...

These studies are primarily concerned with recovery from sublethal actinomycin D or radiation damage as measured by joint lethality of the two treatments separated by an interval of 1 hr to 4 days. In mice given actinomycin D and challenged with radiation, the high mortality attributable to joint treatment decreased steadily between the 6-hr and the 3-day challenge. On the other hand, in mice g...

Actinomycin D has been tested on mouse tu mors, with varying degrees of success, as an anti-neoplastic agent. Dramatic inhibition of some ascites (mouse) tumors has been reported by Sugiura (14) and Gregory et al. (6). Reilly (11) and Sugiura (14) are in agreement that actino-mycin D has slight antineoplastic activity against S-180, and Farber (3) demonstrated antitumor activity against the mal...

The association of actinomycin D and eleven different deoxyribodinucleotides were studied as model complexes for the interaction of actinomycin D and DNA. All of the deoxyribodinucleotides containing guanine will complex with actinomycin D, and the guanine base has a preferred orientation with respect to the chromophore of actinomycin D. The spectrophotometric binding curve for pdG-dC is sigmoi...