The purpose of this study is to determine the relationship between enzymatic antioxidants such as glutathione reductase and s-transferase prolidase adenosine deaminase in patients diagnosed with pancreatic cancer. Prolidase, deaminase, activities were determined spectrophotometrically blood sera collected from healthy control subjects included study. In cancer, mean value enzymes decreased, whi...

The distribution of adenosine deaminase and adenosine deaminase complexing protein in rabbit heart has been compared using immunohistochemical staining procedures. Sections (4-5 microns) of tissue fixed in Clarke's solution or paraformaldehyde and embedded in paraffin were stained by the peroxidase anti-peroxidase method for adenosine deaminase or complexing protein, using affinity purified ant...

Die Bereiche RNA-Modifikation und RNA-Schaden weisen beide eine Vielzahl nicht-kanonischer Nukleosidstrukturen auf. Während sich RNA-Modifikationen zur Verbesserung der RNA-Funktion entwickelt haben, impliziert die Bezeichnung negative Auswirkungen. Auf Grundlage Markierung mit stabilen Isotopen Massenspektrometrie berichten wir von Identifizierung Charakterisierung 2-Methylthio-1,N6-ethenoaden...

Using AMP deaminase (AMP aminohydrolase; EC 3.5.4.6) purified from rabbit left-ventricular heart tissue, we report direct investigation of the potential for cardiac AMP deaminase activity to be regulated by kinase-mediated phosphorylation. Rabbit heart AMP deaminase served as a substrate for Ca2+/phospholipid-dependent protein kinase (protein kinase C; PKC) exclusively; no other mammalian prote...

In many human tissues adenosine deaminase exists as a complex composed of two proteins; one protein has adenosine deaminase activity while the other represents a binding protein with no other known binding activity. A rapid, quantitative assay for human adenosine deaminase binding protein has been developed utilizing ‘251-labeled calf adenosine deaminase. In addition this binding protein has be...

Alterations in the competency of the creatine kinase system elicit numerous structural and metabolic compensations, including changes in purine nucleotide metabolism. We evaluated molecular and kinetic changes in AMP deaminase from skeletal muscles of mice deficient in either cytosolic creatine kinase alone (M-CK-/-) or also deficient in mitochondrial creatine kinase (CK-/-) compared with wild ...

Endostatin, an angiogenesis inhibitor tested in multiple clinical trials, selectively targets neovascular endothelial cells, suppressing tumor growth. To enhance the therapeutic efficacy of endostatin, we fused endostatin with cytosine deaminase, which converts a prodrug 5-flucytosine into a cytotoxic 5-fluorouracil. This therapeutic strategy was developed based on the observation that the endo...

abstract the aim of the present research was to isolate plant growth promoting rhizobacteria producing acc deaminase which was able to alleviate negative effects of salinity on plant growth. 21 composite soil samples and canola roots were collected from the saline and relatively saline soils of qom and qazvin provinces. 105 strains were selected from the soil rhizosphere of canola. according to...

In many human tissues adenosine deaminase exists as a large molecular weight complex (large form) composed of adenosine deaminase and an adenosine deaminase binding protein. The molar ratio of adenosine deaminase to binding protein in this large form complex appears to be 2:1, respectively, based on several observations. Scatchard-type analysis of the binding of 125I-labeled adenosine deaminase...

The chlorovirus PBCV-1, like many large double-stranded DNA-containing viruses, contains several genes that encode putative proteins involved in nucleotide biosynthesis. This report describes the characterization of the PBCV-1 dCMP deaminase, which produces dUMP, a key intermediate in the synthesis of dTTP. As predicted, the recombinant protein has dCMP deaminase activity that is activated by d...