A label-free fluorescence aptasensor was studied for adenosine triphosphate (ATP) detection that combines low biosensor toxicity with a simplified preparation process. In this study, the aptamer of gold nanoparticle@aptamer@carbon quantum dot nanostructure could specifically identify ATP, resulting in change signal. order to analyze performance sensor, effects carbon (CQD) concentration and cen...

A luminescent probe based on a europium complex is developed, which effectively distinguishes adenosine-5'-triphosphate (ATP) from adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in pure water at pH 6.8. With a longer lifetime (in ms range), the probe is prospectively applied to biological systems to monitor ATP levels by completely removing the background fluorescence of other mo...

ATP and several congeners were applied to locus coeruleus neurons in slices cut from rat pons. Whole-cell recording of membrane current showed that ATP caused an inward current at -60 mV. Effective concentrations (applied by superfusion) were 3-300 microM, and the peak current was about 150 pA at -60 mV. 2-Methylthioadenosine 5'-triphosphate was slightly more potent than ATP, adenosine 5'-dipho...

Objective: Erectile dysfunction is a usual side effect of antipsychotic medications; this causes patients to avoid using drugs. The aim study investigate the effects iloperidone, paliperidone and loxapine on serotonin (5-HT), noradrenaline (NA), adenosine triphosphate (ATP) potassium chloride (KCl)-induced contractions vas deferens in mice.Materials Methods: mice were randomly divided into expe...

Bioluminescent analysis of adenosine triphosphate (ATP) concentrations is now acquiring new applications in the form objects and processes which it can be effectively used for sensing. A quick biological systems level ATP one main parameters, a forecast development various situations such biosystems under industrial production conditions or ecological state environment, confirmed by results ana...

An enzyme has been partially purified from brewer's yeast which catalyzes the reversible reaction between riboflavin phosphate and adenosine triphosphate (ATP) on the one hand and flavin-adenine dinucleotide (FAD) and inorganic pyrophosphate on the other in the presence of magnesium ions. ATP could not be replaced by adenosine diphosphate or adenosine-5-phosphate, and in the reverse reaction, i...

Adenosine triphosphate (ATP) is known as an energy source and generated by mitochondria which the powerhouse of cell. The ATP supplies through disassociation phosphate group cellular enzymes. extracellular in environment acts a signaling molecule can act anti-inflammatory molecule, promote cancer progression, also pro-inflammatory molecule. degradation major disadvantage ectonucleotidases that ...

Adenylyl cyclases (ACs) are a special group of enzymes that catalyze formation the second messenger molecule, 3',5'-cyclic adenosine monophosphate (cAMP) from 5'-adenosine triphosphate (ATP). Apparently, even though cAMP is increasingly becoming an important signaling molecule in higher plants, identification plant ACs has somewhat remained slow. Here we report recombinant cloning, partial expr...

The ATP-binding cassette (ABC) transporter, transporter associated with antigen processing (TAP)-like (TAPL) tagged with a histidine cluster was overexpressed, amounting to as much as 1-2% of total membrane proteins in Drosophila cell line S2. TAPL was effectively solubilized from membranes by Triton X-100, NP-40 and n-dodecyl-beta-D-maltoside. Solubilized TAPL bound ATP-agarose and adenosine 5...

The effects of adenine nucleotides and adenosine on DNA synthesis and cell growth have been studied in bovine aortic endothelial cells (BAECs). ATP produced a small but significant (+44%) increase of the fraction of BAECs whose nuclei are labeled by [3H]thymidine. This mitogenic effect was mimicked by ADP, the phosphorothioate analogues ATP gamma S and ADP beta S, and the nonhydrolyzable analog...